Development of a lateral-flow ICA strip for the detection of colchicine

Abstract

Colchicine (COL), which is extracted from colchicine and papaya, is widely used in medicine. However, COL poisoning causes serious adverse complications including death. Therefore, there is a need to develop a sensitive COL detection method. In this study, we developed a highly sensitive monoclonal antibody 1E4 with a half-maximal inhibitory concentration and linear range of 0.43 ng mL⁻¹ and 0.09–2.16 ng mL⁻¹, respectively. Using 1E4, we developed a lateral-flow immunochromatographic assay (ICA) strip for COL detection. Based on the results, the detection interval was 1–25 ng mL⁻¹ in milk, 2.5–50 ng mL⁻¹ in beef, 1–25 ng mL⁻¹ in edible lily and 2.5–25 ng mL⁻¹ in daylily. The lateral-flow ICA strip can be used as an effective tool for COL detection in food samples on site.