Issue 24, 2018

Functional TCR T cell screening using single-cell droplet microfluidics

Abstract

Adoptive T cell transfer, in particular TCR T cell therapy, holds great promise for cancer immunotherapy with encouraging clinical results. However, finding the right TCR T cell clone is a tedious, time-consuming, and costly process. Thus, there is a critical need for single cell technologies to conduct fast and multiplexed functional analyses followed by recovery of the clone of interest. Here, we use droplet microfluidics for functional screening and real-time monitoring of single TCR T cell activation upon recognition of target tumor cells. Notably, our platform includes a tracking system for each clone as well as a sorting procedure with 100% specificity validated by downstream single cell reverse-transcription PCR and sequencing of TCR chains. Our TCR screening prototype will facilitate immunotherapeutic screening and development of T cell therapies.

Graphical abstract: Functional TCR T cell screening using single-cell droplet microfluidics

Supplementary files

Article information

Article type
Paper
Submitted
07 aug 2018
Accepted
26 okt 2018
First published
06 nov 2018

Lab Chip, 2018,18, 3733-3749

Functional TCR T cell screening using single-cell droplet microfluidics

A. I. Segaliny, G. Li, L. Kong, C. Ren, X. Chen, J. K. Wang, D. Baltimore, G. Wu and W. Zhao, Lab Chip, 2018, 18, 3733 DOI: 10.1039/C8LC00818C

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