Issue 9, 2020

Graphene oxide-functionalized nanofibre composite matrices to enhance differentiation of hippocampal neuronal cells

Abstract

During the last decade, graphene-family nanomaterials have been widely utilized, forming a composite with various polymers for potential applications in tissue engineering and regeneration due to their excellent physicochemical characteristics and biocompatibility. Above all, these graphene-family nanomaterials allow polymers to have novel biofunctional properties, while maintaining their intrinsic character. By virtue of this bioactivity of nanocomposites, they are able to modulate cellular behaviours such as cell attachment, proliferation and differentiation. In this study, the nanofibre composite matrices of poly(L-lactide-co-ε-caprolactone) (PLCL) and laminin (Lam) functionalized with graphene oxide (GO) (PLCL/Lam/GO) were prepared via electrospinning to explore whether they have the potential to enhance the differentiation of hippocampal neuronal cells. The PLCL/Lam/GO nanofibre matrices could support the adhesion and proliferation of hippocampal neuronal cells by providing the extracellular matrix-like structure. Furthermore, these PLCL/Lam/GO composite matrices were found to promote neurite outgrowth and alignment from hippocampal neuronal cells through providing optimal microenvironments for neuronal differentiation. The underlying mechanism of this phenomenon was proposed according to the scenario in which neurite outgrowth was guided by biofunctional cues, such as Lam and GO, with nanofibre-mediated topographical cues, which upregulated the expression of some specific genes (i.e., DCX, MAP2 and Nestin) closely related to cytoskeletal interactions. In conclusion, it is suggested that these nanofibre composite matrices can be exploited to craft a range of strategies for the development of novel scaffolds to accelerate neural regeneration.

Graphical abstract: Graphene oxide-functionalized nanofibre composite matrices to enhance differentiation of hippocampal neuronal cells

Supplementary files

Article information

Article type
Paper
Submitted
24 sep 2020
Accepted
31 okt 2020
First published
02 nov 2020
This article is Open Access
Creative Commons BY-NC license

Mater. Adv., 2020,1, 3496-3506

Graphene oxide-functionalized nanofibre composite matrices to enhance differentiation of hippocampal neuronal cells

M. S. Kang, J. H. Lee, S. Song, D. Shin, J. Jang, S. Hyon, S. W. Hong, J. H. Lee and D. Han, Mater. Adv., 2020, 1, 3496 DOI: 10.1039/D0MA00733A

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements