Issue 71, 2019

2′-Fluoro-c-di-GMP as an oral vaccine adjuvant

Abstract

Bis-(3′–5′)-cyclic dimeric 2′-deoxy-2′-fluoroguanosine monophosphate (2′-F-c-di-GMP) was synthesized through the modified H-phosphonate chemistry. Oral immunization of C57BL/6 mice with Helicobacter pylori cell-free sonicate extract adjuvanted with 2′-F-c-di-GMP led to the production of antigen-specific antibodies in feces and sera, and lowered bacterial counts in the stomach upon post-vaccination infections in immunized mice. Similarly, oral vaccination of BALB/c mice with flagillin proteins from Clostridium difficile and Listeria monocytogenes adjuvanted with 2′-F-c-di-GMP led to production of antigen-specific antibodies both systemically and mucosally. The adjuvanticity of 2′-F-c-di-GMP is associated with the enhanced induction of interferon γ. These results demonstrated the excellent oral adjuvanticity of 2′-F-c-di-GMP.

Graphical abstract: 2′-Fluoro-c-di-GMP as an oral vaccine adjuvant

Supplementary files

Article information

Article type
Paper
Submitted
12 okt 2019
Accepted
05 dec 2019
First published
16 dec 2019
This article is Open Access
Creative Commons BY license

RSC Adv., 2019,9, 41481-41489

2′-Fluoro-c-di-GMP as an oral vaccine adjuvant

J. Li, R. K. Lee, W. Chen and H. Yan, RSC Adv., 2019, 9, 41481 DOI: 10.1039/C9RA08310C

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