Themed collection Most popular 2022 analytical chemistry articles
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State-of-the-art accounts of hyperpolarized 15N-labeled molecular imaging probes for magnetic resonance spectroscopy and imaging
This review presents a current account of hyperpolarized 15N-labeled molecular imaging probes, as well as insights on their advantages and challenges to advance future development of 15N-based probes and their applications in MRS/MRI.
Chem. Sci., 2022,13, 7378-7391
https://doi.org/10.1039/D2SC01264B
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An antibody scanning method for the detection of α-synuclein oligomers in the serum of Parkinson's disease patients
Misfolded α-synuclein oligomers are closely implicated in the pathology of Parkinson's disease and related synucleinopathies.
Chem. Sci., 2022,13, 13815-13828
https://doi.org/10.1039/D2SC00066K
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Ultrasensitive chemiluminescent neuraminidase probe for rapid screening and identification of small-molecules with antiviral activity against influenza A virus in mammalian cells
A new chemiluminescence neuraminidase probe enables rapid screening of small molecules that inhibit viral replication, directly in influenza A-infected mammalian cells.
Chem. Sci., 2022,13, 12348-12357
https://doi.org/10.1039/D2SC03460C
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Imaging of hypochlorous acid in mitochondria using an asymmetric near-infrared fluorescent probe with large Stokes shift
Small-molecule near-infrared (NIR) imaging facilitates deep tissue penetration, low autofluorescence, non-invasive visualization, and a relatively simple operation.
Chem. Sci., 2022,13, 11140-11149
https://doi.org/10.1039/D2SC03833A
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In situ monitoring of functional activity of extracellular matrix stiffness-dependent multidrug resistance protein 1 using scanning electrochemical microscopy
SECM using FcCOOH as the redox mediator and endogenous GSH as the indicator was employed to investigate the effect of extracellular matrix stiffness on the functional activity of MRP1 in cancer cells in situ.
Chem. Sci., 2022,13, 10349-10360
https://doi.org/10.1039/D2SC02708A
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Simultaneous Raman and infrared spectroscopy: a novel combination for studying bacterial infections at the single cell level
O-PTIR was used for simultaneous collection of infrared and Raman spectra from clinical pathogens associated with bloodstream infections.
Chem. Sci., 2022,13, 8171-8179
https://doi.org/10.1039/D2SC02493D
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Mapping paratopes of nanobodies using native mass spectrometry and ultraviolet photodissociation
Ultraviolet photodissociation mass spectrometry is used to probe the paratopes of nanobodies, a class of single-domain antibodies, and to determine intersubunit salt-bridges and explore the nanobody·antigen interfaces.
Chem. Sci., 2022,13, 6610-6618
https://doi.org/10.1039/D2SC01536F
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Understanding of chiral site-dependent enantioselective identification on a plasmon-free semiconductor based SERS substrate
An intelligent enantioselective identification strategy is designed to demonstrate that both enantioselectivity and stereoselectivity are crucial factors for chiral sensing.
Chem. Sci., 2022,13, 6550-6557
https://doi.org/10.1039/D2SC01938H
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Standard-free single magnetic bead evaluation: a stable nanoplatform for prostate disease differentiation
A standard-free stable single magnetic bead nanoplatform was proposed in this work. The use of metal signal ratio was directly applied for intrinsic biological fPSA to tPSA ratio evaluations for prostate disease differentiation.
Chem. Sci., 2022,13, 6270-6275
https://doi.org/10.1039/D2SC00928E
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Silicon functionalization expands the repertoire of Si-rhodamine fluorescent probes
Functionalization of the bridging silicon atom of Si-rhodamine dyes allows tuning of dye performance, the attachment of sensors, and the addition of biomolecular targeting ligands useful for the construction of live cell imaging probes.
Chem. Sci., 2022,13, 6081-6088
https://doi.org/10.1039/D2SC01821G
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Detection of cannabinoid receptor type 2 in native cells and zebrafish with a highly potent, cell-permeable fluorescent probe
Detection and visualization of the cannabinoid receptor type 2 by a cell-permeable high affinity fluorescent probe platform enables tracing receptor trafficking in live cells and in zebrafish.
Chem. Sci., 2022,13, 5539-5545
https://doi.org/10.1039/D1SC06659E
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A dynamic DNA nanosponge for triggered amplification of gene-photodynamic modulation
Oligonucleotide drug delivery approach is provided with a biomimetic, dynamic DNA nanomaterial, which enables disease gene regulation and auxiliary therapy in a controllable and self-boosting manner.
Chem. Sci., 2022,13, 5155-5163
https://doi.org/10.1039/D2SC00459C
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Reversible inhibition of the oxidase-like activity of Fe single-atom nanozymes for drug detection
Inhibitory effects of paracetamol on the oxidase-like activity of Fe single-atom nanozymes.
Chem. Sci., 2022,13, 4566-4572
https://doi.org/10.1039/D2SC00212D
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Molecularly imprinted polymers outperform lectin counterparts and enable more precise cancer diagnosis
A triple molecularly imprinted polymer (MIP)-based plasmonic assay was developed for precise cancer diagnosis in terms of the relative glycosylation expression of glycoprotein biomarkers.
Chem. Sci., 2022,13, 4589-4597
https://doi.org/10.1039/D2SC01093C
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A caged imidazopyrazinone for selective bioluminescence detection of labile extracellular copper(II)
A caged imidazopyrazinone can detect reactive extracellular copper in mammalian systems using bioluminescence generated with the ATP-independent nanoluciferase enzyme.
Chem. Sci., 2022,13, 4352-4363
https://doi.org/10.1039/D1SC07177G
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MnO2 nanosheets as a carrier and accelerator for improved live-cell biosensing application of CRISPR/Cas12a
Herein, we demonstrate that MnO2 nanosheets are an excellent carrier of CRISPR/Cas12a due to the two important roles played by them.
Chem. Sci., 2022,13, 4364-4371
https://doi.org/10.1039/D1SC06383A
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A two-photon ratiometric fluorescent probe for real-time imaging and quantification of NO in neural stem cells during activation regulation
Developing a novel tool capable of real-time monitoring and accurate quantification of NO is critical to understanding its role in physiological and pathological processes.
Chem. Sci., 2022,13, 4303-4312
https://doi.org/10.1039/D2SC00326K
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Profiling single-molecule reaction kinetics under nanopore confinement
A four-state kinetics model is proposed to reveal the kinetics of a single-molecule reaction under nanopore confinement.
Chem. Sci., 2022,13, 4109-4114
https://doi.org/10.1039/D1SC06837G
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Unique assembly of carbonylpyridinium and chromene reveals mitochondrial thiol starvation under ferroptosis and novel ferroptosis inducer
Carbonylpyridinium and chromene were elaborately assembled to highly target mitochondrial thiol assay by releasing o-quinone methide from CM-Mit to label proteins, thus avoiding diffusion out of the mitochondria, which enabled accurately spatiotemporal detection of thiol.
Chem. Sci., 2022,13, 3706-3712
https://doi.org/10.1039/D2SC00328G
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New 19F NMR methodology reveals structures of molecules in complex mixtures of fluorinated compounds
19F-centred NMR structure determination protocol alleviates the need for compound separation. Disinfection byproducts of chloramination were unraveled by analyzing the reaction pathways of a single fluorinated molecule.
Chem. Sci., 2022,13, 3766-3774
https://doi.org/10.1039/D1SC06057K
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Stimulated Raman scattering microscopy with spectral phasor analysis: applications in assessing drug–cell interactions
Stimulated Raman scattering microscopy with spectral phasor analysis provides a label-free approach for phenotypic evaluation of drug-induced effects.
Chem. Sci., 2022,13, 3468-3476
https://doi.org/10.1039/D1SC06976D
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Near-infrared fluorescent probe for hydrogen sulfide: high-fidelity ferroptosis evaluation in vivo during stroke
A H2S triggered and H2S releasing near-infrared fluorescent probe (HL-H2S) was developed. HL-H2S does not interfere with the progression of ferroptosis by consuming H2S, thus enabling high-fidelity in situ imaging of ferroptosis.
Chem. Sci., 2022,13, 2992-3001
https://doi.org/10.1039/D1SC05930K
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Precise and long-term tracking of mitochondria in neurons using a bioconjugatable and photostable AIE luminogen
A novel bioconjugatable and photostable AIE luminogen has been rationally synthesized for precise and long-term tracking of neuron mitochondria.
Chem. Sci., 2022,13, 2965-2970
https://doi.org/10.1039/D1SC06336G
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On the formation of hydrogen peroxide in water microdroplets
A. Gallo Jr, H. Mishra et al., pinpoint the origins of the spontaneous H2O2 formation in water microdroplets formed via spraying or condensation, i.e., without the addition of electrical energy, catalyst, or co-solvent.
Chem. Sci., 2022,13, 2574-2583
https://doi.org/10.1039/D1SC06465G
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Solid-state 17O NMR study of α-D-glucose: exploring new frontiers in isotopic labeling, sensitivity enhancement, and NMR crystallography
We report the first “total synthesis” of 17O-labeled D-glucose and its solid-state 17O NMR characterization with unprecedented sensitivity and resolution.
Chem. Sci., 2022,13, 2591-2603
https://doi.org/10.1039/D1SC06060K
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A highly selective and sensitive chemiluminescent probe for leucine aminopeptidase detection in vitro, in vivo and in human liver cancer tissue
The first chemiluminescent probe for the detection of LAP is described. It shows a highly selective, sensitive and rapid chemiluminescence response for the detection of LAP in vitro and in vivo, and enables the differentiation of liver cancer.
Chem. Sci., 2022,13, 2324-2330
https://doi.org/10.1039/D1SC06528A
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2′-O-Methyl modified guide RNA promotes the single nucleotide polymorphism (SNP) discrimination ability of CRISPR–Cas12a systems
This study illustrates that 2′-O-methyl modified gRNAs improve the specificity of the CRISPR–Cas12a system (mg-CRISPR) via suppressing the Cas12a's affinity to off-target DNA and provides an efficient strategy for high-specificity gRNA design.
Chem. Sci., 2022,13, 2050-2061
https://doi.org/10.1039/D1SC06832F
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PAM-less conditional DNA substrates leverage trans-cleavage of CRISPR-Cas12a for versatile live-cell biosensing
This work developed the PAM-less conditional DNA substrates that leverage the trans-cleavage effect of CRISPR-Cas12a to sense various biomolecules in living cells.
Chem. Sci., 2022,13, 2011-2020
https://doi.org/10.1039/D1SC05558E
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Dynamic metabolic change of cancer cells induced by natural killer cells at the single-cell level studied by label-free mass cytometry
Label-free mass cytometry is utilized to study the dynamic metabolic change during apoptosis in HepG2 cells induced by NK92 MI cells at the single-cell level. The metabolic heterogeneity of individual HepG2 cells during apoptosis was revealed.
Chem. Sci., 2022,13, 1641-1647
https://doi.org/10.1039/D1SC06366A
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An “OFF–ON–OFF” fluorescence protein-labeling probe for real-time visualization of the degradation of short-lived proteins in cellular systems
An “OFF–ON–OFF” fluorescence probe for real-time imaging of the expression (fluorescence ‘OFF’) and degradation (fluorescence ‘ON’) of short lived PYP-tag proteins in cellular systems.
Chem. Sci., 2022,13, 1419-1427
https://doi.org/10.1039/D1SC06274C
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