Issue 29, 2020

Thioglycoligation of aromatic thiols using a natural glucuronide donor

Abstract

The β-D-glucuronidase DtGlcA from Dictyoglomus thermophilum was engineered to generate an active thioglycoligase that is able to catalyse the formation of numerous S-glucuronides. Its X-ray structure analysis indicated the ability of the biocatalyst to bind aromatic thiol acceptors for S-glycosylation. Noteworthily, the DtGlcA mutant was found to be the first thioligase that is able to use a natural sugar donor different from the widely used synthetic para-nitrophenyl glycosides.

Graphical abstract: Thioglycoligation of aromatic thiols using a natural glucuronide donor

Supplementary files

Article information

Article type
Communication
Submitted
03 Febr. 2020
Accepted
07 Jūl. 2020
First published
07 Jūl. 2020

Org. Biomol. Chem., 2020,18, 5582-5585

Thioglycoligation of aromatic thiols using a natural glucuronide donor

M. Kurdziel, M. Kopeć, A. Pâris, K. Lewiński, P. Lafite and R. Daniellou, Org. Biomol. Chem., 2020, 18, 5582 DOI: 10.1039/D0OB00226G

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