Issue 31, 2021

Label-free methods for optical in vitro characterization of protein–protein interactions

Abstract

Protein–protein interactions are involved in the regulation and function of the majority of cellular processes. As a result, much effort has been aimed at the development of methodologies capable of quantifying protein–protein interactions, with label-free methods being of particular interest due to the associated simplified workflows and minimisation of label-induced perturbations. Here, we review recent advances in optical technologies providing label-free in vitro measurements of affinities and kinetics. We provide an overview and comparison of existing techniques and their principles, discussing advantages, limitations, and recent applications.

Graphical abstract: Label-free methods for optical in vitro characterization of protein–protein interactions

Article information

Article type
Perspective
Submitted
10 Marts 2021
Accepted
23 Jūl. 2021
First published
03 Aug. 2021
This article is Open Access
Creative Commons BY license

Phys. Chem. Chem. Phys., 2021,23, 16488-16500

Label-free methods for optical in vitro characterization of protein–protein interactions

F. Soltermann, W. B. Struwe and P. Kukura, Phys. Chem. Chem. Phys., 2021, 23, 16488 DOI: 10.1039/D1CP01072G

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