Issue 6, 2021

An overview of methods for the structural and functional mapping of epitopes recognized by anti-SARS-CoV-2 antibodies

Abstract

This mini-review presents a critical survey of techniques used for epitope mapping on the SARS-CoV-2 Spike protein. The sequence and structures for common neutralizing and non-neutralizing epitopes on the Spike protein are described as determined by X-ray crystallography, electron microscopy and linear peptide epitope mapping, among other methods. An additional focus of this mini-review is an analytical appraisal of different deep mutational scanning workflows for conformational epitope mapping and identification of mutants on the Spike protein which escape antibody neutralization. Such a focus is necessary as a critical review of deep mutational scanning for conformational epitope mapping has not been published. A perspective is presented on the use of different epitope determination methods for development of broadly potent antibody therapies and vaccines against SARS-CoV-2.

Graphical abstract: An overview of methods for the structural and functional mapping of epitopes recognized by anti-SARS-CoV-2 antibodies

Article information

Article type
Review Article
Submitted
19 Aug. 2021
Accepted
25 Sept. 2021
First published
29 Sept. 2021
This article is Open Access
Creative Commons BY-NC license

RSC Chem. Biol., 2021,2, 1580-1589

An overview of methods for the structural and functional mapping of epitopes recognized by anti-SARS-CoV-2 antibodies

I. M. Francino-Urdaniz and T. A. Whitehead, RSC Chem. Biol., 2021, 2, 1580 DOI: 10.1039/D1CB00169H

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