Target identification of mouse stem cell probe CDy1 as ALDH2 and Abcb1b through live-cell affinity-matrix and ABC CRISPRa library

Naoki Miyamoto; Young-Hyun Go; Larissa Miasiro Ciaramicoli; Haw-Young Kwon; Heon Seok Kim; Xuezhi Bi; Young Hyun Yu; Beomsue Kim; Hyung-Ho Ha; Nam-Young Kang; Seong-Wook Yun; Jin-Soo Kim; Hyuk-Jin Cha; Young-Tae Chang

doi:10.1039/D1CB00147G

Target identification of mouse stem cell probe CDy1 as ALDH2 and Abcb1b through live-cell affinity-matrix and ABC CRISPRa library†

Naoki Miyamoto,‡^a Young-Hyun Go,‡^b Larissa Miasiro Ciaramicoli,‡^c Haw-Young Kwon,^a Heon Seok Kim,^d Xuezhi Bi,^e Young Hyun Yu,^f Beomsue Kim,

^g Hyung-Ho Ha,^f Nam-Young Kang,^h Seong-Wook Yun,ⁱ Jin-Soo Kim,^jk Hyuk-Jin Cha*^l and Young-Tae Chang

*^ac

Author affiliations

* Corresponding authors

^a Center for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang 37673, Republic of Korea
E-mail: ytchang@postech.ac.kr

^b Department of Life Science, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 04107, South Korea

^c Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk 37673, Republic of Korea

^d Division of Oncology, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA

^e Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Singapore 138668, Singapore

^f College of Pharmacy, Sunchon National University, Jungangro 255, Sunchon 57922, South Korea

^g Neural Circuit Research Group, Korea Brain Research Institute (KBRI), Daegu, Republic of Korea

^h Department of Convergence IT Engineering, Pohang University of Science and Technology, Pohang, Gyeongbuk 37673, Korea

ⁱ Nonclinical Drug Safety, Boehringer Ingelheim Pharma GmbH & Co. KG, 88397 Biberach an der Riss, Germany

^j Department of Chemistry, Seoul National University, Seoul 08826, Republic of Korea

^k Center for Genome Engineering, Institute for Basic Science (IBS), Daejeon 34126, Republic of Korea

^l College of Pharmacy, Seoul National University, Seoul, Republic of Korea
E-mail: hjcha93@snu.ac.kr

Abstract

CDy1 is a powerful tool to distingusih embryonic stem cells for reprogramming studies and regeneration medicine. However, the stem cell selectivity mechanism of CDy1 has not been fully understood. Here, we report ALDH2 and ABCB1 as the molecular targets of CDy1, elucidated by live-cell affinity-matrix and ABC transporter CRISPRa library screening. The two unique orthogonal mechanisms provide the potential of multi-demensional cellular distinction of specific cell types.

This article is part of the themed collection: Small molecules for chemical biology

RSC Chemical Biology

Target identification of mouse stem cell probe CDy1 as ALDH2 and Abcb1b through live-cell affinity-matrix and ABC CRISPRa library†

Abstract

Supplementary files

Article information

Download Citation

Permissions

Target identification of mouse stem cell probe CDy1 as ALDH2 and Abcb1b through live-cell affinity-matrix and ABC CRISPRa library

Social activity

Search articles by author

Spotlight

Advertisements