Issue 22, 2015

Selective choline biosensors based on choline oxidase co-immobilized into self-assembled monolayers on micro-chips at low potential

Abstract

We fabricate a choline-biosensor that exhibits excellent specific and selective recognition for selected biological molecules coexisting with other analytes in a buffer system at low potential. This novel sensor is a well-organized design with the enzyme choline oxidase (ChOx) immobilized onto a micro-chip for selective choline (Ch) detection. This reusable and sensitive Ch biosensor is developed based on mediator-free ChOx self-assembled monolayers (SAM) on a micro-chip. Cyclic voltammetry (CV) technique was employed with the enzyme fabricated chips in phosphate buffer solution (PBS, 0.1 M) under room conditions. The analytical parameters of ChOx fabricated electrode displayed a lower detection limit (DL, 0.012 ± 0.005 nM), a wide linear dynamic range (LDR, 0.05 nM to 10.0 μM), good linearity (R = 0.9938), and higher sensitivity (3.5 μA μM−1 cm−2), where only a small sample volume (50.0 μL) was analyzed. The micro-chip system exhibits a simple and efficient approach to immobilize oxidative enzymes onto thioglycolic acid (TGA) SAM modified surfaces, which could improve the biosensor detection of a large group of biomolecules for a broad range of biomedical applications in health care. This integrated micro-chip provides a promising low-cost platform for the sensitive and rapid detection of biomolecules using small samples.

Graphical abstract: Selective choline biosensors based on choline oxidase co-immobilized into self-assembled monolayers on micro-chips at low potential

Article information

Article type
Paper
Submitted
15 Sept. 2015
Accepted
30 Sept. 2015
First published
08 Okt. 2015

Anal. Methods, 2015,7, 9426-9434

Author version available

Selective choline biosensors based on choline oxidase co-immobilized into self-assembled monolayers on micro-chips at low potential

M. M. Rahman and A. M. Asiri, Anal. Methods, 2015, 7, 9426 DOI: 10.1039/C5AY02456K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements