Issue 9, 2020

Smartphone-based multiplex 30-minute nucleic acid test of live virus from nasal swab extract

Abstract

Rapid, sensitive and specific detection and reporting of infectious pathogens is important for patient management and epidemic surveillance. We demonstrated a point-of-care system integrated with a smartphone for detecting live virus from nasal swab media, using a panel of equine respiratory infectious diseases as a model system for corresponding human diseases such as COVID-19. Specific nucleic acid sequences of five pathogens were amplified by loop-mediated isothermal amplification on a microfluidic chip and detected at the end of reactions by the smartphone. Pathogen-spiked horse nasal swab samples were correctly diagnosed using our system, with a limit of detection comparable to that of the traditional lab-based test, polymerase chain reaction, with results achieved in ∼30 minutes.

Graphical abstract: Smartphone-based multiplex 30-minute nucleic acid test of live virus from nasal swab extract

Supplementary files

Article information

Article type
Paper
Submitted
25 Marts 2020
Accepted
17 Apr. 2020
First published
21 Apr. 2020

Lab Chip, 2020,20, 1621-1627

Author version available

Smartphone-based multiplex 30-minute nucleic acid test of live virus from nasal swab extract

F. Sun, A. Ganguli, J. Nguyen, R. Brisbin, K. Shanmugam, D. L. Hirschberg, M. B. Wheeler, R. Bashir, D. M. Nash and B. T. Cunningham, Lab Chip, 2020, 20, 1621 DOI: 10.1039/D0LC00304B

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