Issue 15, 2017

A two-photon fluorescent probe for viscosity imaging in vivo

Abstract

A new two-photon fluorescent probe (MCN) for viscosity imaging was developed based on a 6-substituted quinoline framework. MCN showed an excellent “off–on” fluorescence response (ca. 90-fold enhancement) with viscosity increasing in the glycerol–water viscosity system. MCN showed great sensitivity to viscosity (R2 = 0.98, x = 0.65), which gave rise to cell imaging for micro-viscosity or real-time cell imaging during apoptosis with low cytotoxicity under two-photon excitation (λex = 800 nm). Fluorescence lifetime imaging (FLIM) of living HeLa cells stained with MCN revealed that the intracellular average viscosity value was 73.45 ± 21.55 cP in cytosol. Imaging in living tissue slices indicated that MCN can work in deep tissue (∼130 μM) under two-photon excitation. Moreover, MCN also showed the capacity for in vivo imaging viscosity in zebrafish with obvious fluorescence emission.

Graphical abstract: A two-photon fluorescent probe for viscosity imaging in vivo

Supplementary files

Article information

Article type
Paper
Submitted
12 Janv. 2017
Accepted
25 Febr. 2017
First published
27 Febr. 2017

J. Mater. Chem. B, 2017,5, 2743-2749

A two-photon fluorescent probe for viscosity imaging in vivo

P. Ning, P. Dong, Q. Geng, L. Bai, Y. Ding, X. Tian, R. Shao, L. Li and X. Meng, J. Mater. Chem. B, 2017, 5, 2743 DOI: 10.1039/C7TB00136C

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