Issue 91, 2021

Visualization of lipophagy using a supramolecular FRET pair

Abstract

A rationally designed supramolecular FRET pair consisting of cyanine3-cucurbit[7]uril (Cy3-CB[7]) and boron-dipyrromethene 630/650-adamantylammonium (BDP-AdA) can be used to visualize organelle-specific autophagy events. The intracellular accumulations of Cy3-CB[7] in lysosomes and BDP-AdA in lipid droplets (LDs) and the formation of an intracellular host–guest complex between Cy3-CB[7] and BDP-AdA resulting in FRET signals allow us to visualize the fusion of LDs with lysosomes, namely, lipophagy. This study demonstrates the potential of supramolecular imaging based on bio-orthogonal host–guest interactions in the investigation of selective autophagy events.

Graphical abstract: Visualization of lipophagy using a supramolecular FRET pair

Supplementary files

Article information

Article type
Communication
Submitted
27 8 2021
Accepted
26 10 2021
First published
27 10 2021

Chem. Commun., 2021,57, 12179-12182

Visualization of lipophagy using a supramolecular FRET pair

A. Lee, M. Li, Y. H. Ko, S. Park, J. Seo, K. M. Park and K. Kim, Chem. Commun., 2021, 57, 12179 DOI: 10.1039/D1CC04779E

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