Traditional uses, phytochemistry, pharmacology, and toxicology of Pterocephalus hookeri (C. B. Clarke) Höeck: a review

Abstract

Pterocephalus hookeri (C. B. Clarke) Höeck is a member of the Dipsacaceae family and has been used in traditional Tibetan medicine for thousands of years. P. hookeri clears heat, detoxifies, stops dysentery, eliminates distemper, dispels wind, and relieves stagnation and is mainly prescribed for heat syndrome, dysentery, arthritis, and plague. Approximately 93 chemical compounds have been isolated and identified from P. hookeri, including iridoid glycosides, lignan and triterpenoids. Meanwhile, modern pharmacological studies have shown that P. hookeri has anti-inflammatory, anti-rheumatoid arthritis, analgesic, anticancer, and neuroprotection activities. However, studies on the in vivo pharmacokinetics and mechanism of action, discovery of quality markers, and qualitative and quantitative analysis are still insufficient. Hence, this paper provides a comprehensive review of the ethnic medicine, phytochemistry, pharmacology, and toxicology of P. hookeri to increase the understanding of the medicinal value of P. hookeri.

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Zhiqiang Gan

Zhiqiang Gan, male, studied at the School of Pharmacy, Chengdu University of TCM. From 2019 to present, he has been studying for a Master's degree in the chemistry of traditional Chinese medicine, under the tutelage of Dr Zhang Yi, the director of The Academic Inheritance and Innovation Research Center of Ethnic Medicine of Chengdu University of TCM. The research direction is the effective material basis of Chinese medicine and ethnic medicine.

Juan Jiang

Juan Jiang, M. Sc, attending doctor. She graduated from Chongqing Medical University with a Master's degree in 2014. Since then, she has been working in the Chongqing University Cancer Hospital. She is currently an attending doctor in the Department of Traditional Chinese Medicine and has published many papers in Chinese journals. Her research direction is acupuncture.

Xianli Meng

Xianli Meng, PhD, Professor, is the Dean of the Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of TCM. Professor Meng is the academic and technical leader of Sichuan Province and the review expert of National Natural Science Foundation of China evaluation. She has published more than 50 research papers in various SCI journals. As the leader of the team, Dr Meng is committed to the pharmacodynamics of traditional Chinese medicine and the application of NIR-II in the diagnosis of diseases.

Jia Yu

Jia Yu, lecturer, currently working at the School of Ethnic Medicine, Chengdu University of TCM. From 2019 to present, studying for a PhD, degree under the guidance of Dr Zhang Yi of Chengdu University of TCM. She has undertaken a number of scientific research projects and published more than 10 research papers in Chinese journals, and attended international and domestic conferences. Her current research direction is ethnic medicine and data informatization.

Yi Zhang

Yi Zhang, PhD, researcher, chief Professor of Chengdu University of TCM, PhD, and M.Sc., tutor, is the director of The Academic Inheritance and Innovation Research Center of Ethnic Medicine of Chengdu University of TCM. At present, he has published more than 200 academic papers. As the leader of the team, he is committed to the modernization of traditional Chinese medicine and ethnic medicine, and his main research direction is the effective material basis and quality control of traditional Chinese medicine and ethnic medicine.

Ce Tang

Ce Tang, PhD, post-doctorate, assistant research fellow, currently working at the School of Ethnic Medicine, Chengdu University of TCM. He has undertaken more than 20 projects and published more than 30 research papers, including more than 10 papers in science magazine journals. His research direction is the pharmacodynamic material basis of TCM/ethnic medicine and the development of the key technology of mass spectrometry imaging for the complex traditional Chinese medicine/ethnic medicine system.

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1. Introduction

Pterocephalus hookeri (C. B. Clarke) Höeck is an herbaceous plant that belongs to the subfamily Pterocephalus in the Dipsacaceae family. The genus Pterocephalus currently includes 25 species which are mainly distributed in Europe, Asia, and Africa. Two species, P. hookeri and Pterocephalus bretschneideri (Batalin) E. Pritz. ex Diels, are found in China. P. hookeri is widely distributed in Sichuan, Yunnan, and Tibet (China)^1,2 and is one of the most popular Traditional Tibetan Medicines, known as “”. It is known as fairy grass and is recorded in many classic Tibetan medicine books, such as The Four Medical Tantras (late 8th century AD)³ and Jing Zhu Materia Medica (19th century AD).⁴ The formal Chinese name of P. hookeri is Yi-shou-cao (Chinese name: ), which was first adopted by the Chinese Pharmacopoeia in 1977.⁵ P. hookeri is also known as Bang-zi-du-wu (Tibetan name: ) in traditional Tibetan medicine and is widely used to treat cold, pain, plagues, and arthritis. Since 1993, the phytochemical and pharmacological research of P. hookeri has attracted widespread attention.⁶ To date, compounds isolated from P. hookeri, include: iridoids, triterpenoids, and phenylpropanoids. Modern pharmacological studies have demonstrated that P. hookeri possesses anti-inflammatory, anti-rheumatoid arthritis, analgesic, antitumor, immunomodulatory, neuroprotection, and antibacterial activities.

This review aims to summarize comprehensive information on the botanical characteristics, distribution, traditional use, ethnopharmacology, chemical composition, pharmacological activity, and toxicity characteristics of P. hookeri, referencing ancient books and modern documents, to lay the foundation for further research on the mechanism of action of this traditional medicinal plant and to guide the development of therapeutic drugs.

2. Botanical characterization and distribution

P. hookeri is a perennial herb with a height of 5–35 cm. Its roots are conical, thick, and fleshy, and the upper part of the root is densely covered with brown residual petioles. Their leaves are basal, spatulate to oblong-oblanceolate, 3–20 cm long, and 1–4 cm wide, have curved coarsely dentate or pinnately lobed tips, and are tapered into stalks at the base, and have conspicuous midribs. Both sides of their leaves have thick and short villi (hairs). The scape is single, rarely two or three, and densely shirred. The capitulum is spherical, with a diameter of 2–3 cm. The flowers are white or pink, and the bracts are like involucres, narrow. The epicalyx is tube-shaped, approximately 5 mm long, and pilose. The calyx is completely split into a pinnate crest. The corolla is funnel shaped, and 8–12 mm long. The apex has four or five lobes, and the crown tube is pubescent (fine short hairs) inside and outside. The stamens, usually four in number, are slightly protruding. The flowering and fruit period of P. hookeri is from July to September each year. The habitat, the whole plant, and the inflorescence of P. hookeri are shown in Fig. 1.

Fig. 1 Photographs of the original plant of P. hookeri in its natural habitat, the whole plant, and its flowers.

P. hookeri mainly grows on hillsides, grasslands, meadows, and forests at an altitude of 1800–5700 m. In China, it is mainly distributed in eastern Tibet, northwestern Yunnan, southern Qinghai, southern Gansu, and northwestern Sichuan. In addition, P. hookeri can also be found in Nepal, Sikkim, Bhutan, and northern India.⁷

3. Traditional medicinal uses

P. hookeri is mostly used in China and Tibet as a traditional Tibetan medicine, with a long history of its use as a drug. In traditional Tibetan medicine, the main effect of P. hookeri is to clear heat, detoxify, dispel wind, and relieve pain. It is mainly used to treat exogenous fever and rheumatoid arthritis.⁴ In the early stages of preparing this paper, the research group referred to Tibetan medicine-related monographs, woodcut boards, and handwritten documents, which confirmed that the earliest record of the application of P. hookeri is the Dunhuang Tubo medical document “Chang Juan” (),⁸ which was written before the 7th century AD. Many other ancient Tibetan medical classics, such as “The Four Medical Tantras” (),³ “Jing Zhu Materia Medica” (),⁴ and “Lan Liu Li” (),⁹ also recorded the uses of P. hookeri. Moreover, the herb has been included in many standards, such as the Chinese Pharmacopoeia),¹⁰ Pharmacopoeia standards of the Ministry of Health,¹¹ and Tibetan medicine standards¹² (Fig. 2). In a similar way to traditional Chinese medicine, P. hookeri is used to treat diseases in the form of prescriptions. According to the research team's preliminary statistics, 215 prepared preparations containing P. hookeri are available, which are mostly used in combination with Corydalis hendersonii, Corydalis mucronifera, Gentiana straminea, and Terminalia chebula for the treatment of plague, pneumonia, colds, measles, biliary fever, intestinal fever, and arthritis.¹³ Some of them have withstood the test of time and are still used by the Chinese Tibetan hospitals, and some have even become mature medicines, occupying a certain place in the market (Table 1). In summary, P. hookeri and its prescriptions are mainly used to treat inflammatory and pain-related disease.

Fig. 2 The important records of P. hookeri.

Table 1 Commercial drugs containing P. hookeri used in China

No	Preparation name	Composition	Traditional and clinical uses	Ref.
1	Shi'erwei Yishou San	Pterocephali herba, Santali albi lignum, Carthami flos, and so on	Treatment of plague, influenza, Japanese encephalitis, fever	10
2	Jiebai Wan	Pterocephali herba, Carthami flos, Myristicae semen, and so on	Treatment of indigestion, stomach pain, vomiting and diarrhea	10
3	Pomegranate Puan San	Pterocephali herba, Cinnamomi cortex, Piperis longi fructus, and so on	Treatment of indigestion, urination problems, stomach pain	11
4	Jiuwei QingPeng San	Pterocephali herba, Radix Inulae racemosa, Chebulae fructus, and so on	Treatment of pneumonia, fever, sore throat	11
5	Dasimabao Wan	Pterocephali herba, Chebulae fructus, Aucklandiae radix, and so on	Treatment of meningitis, colds, pharyngitis, pneumonia	11
6	Qingfei Zhike Wan	Pterocephali herba, Arnebiae radix, Phyllanthi fructus, and so on	Treatment of lung disease, colds, cough, chest pain	11
7	Ershiwuwei Yuganzi San	Pterocephali herba, Dendrobii caulis, Adhatoda vasica Nees, and so on	Treatment of high blood pressure, stomach ulcers, liver pain	11
8	Ershiwuwei Yuganzi Wan	Pterocephali herba, Carthami flos, Aucklandiae radix, and so on	Treatment of high blood pressure, liver pain, thirst, irregular menstruation	11
9	Shierwei Qixiao Tangsan	Pterocephali herba, Gentianae macrophullae flos, Solms-laubachiae radix, and so on	Treatment of cough, influenza	11
10	Jiedu capsule	Pterocephali herba, Cistanches herba, Tsaoko fructus, and so on	Treatment of dermatitis and other skin diseases	14

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4. Phytochemistry

To date, 93 phytoconstituents have been isolated from the different parts of P. hookeri, including iridoids, triterpenes, fatty acids, lignans, flavonoids, steroids, saccharides, and amino acids (Table 2).

Table 2 Chemical composition of P. hookeri

No.	Compounds	Molecular	Type	Plant part	Ref.
1	Loganin	C17H26O10	Iridoid	Aerial regions, whole plant	15–20
2	Loganetin	C11H16O5	Iridoid	Aerial regions	16
3	Loganic acid	C16H24O10	Iridoid	Whole plant	18 and 20
4	Isoboonein	C9H14O3	Iridoid	Root	21 and 22
5	Sweroside	C16H22O9	Iridoid (seco-iridoid)	Root, whole plant	16–22
6	6′-Apiofuranosylsweroside	C21H30O13	Iridoid (seco-iridoid)	Whole plant	20
7	Pterocenoid B	C22H30O10	Iridoid (bis-iridoid)	Root	23
8	Pterocenoid C	C22H30O10	Iridoid (bis-iridoid)	Root	20
9	Pterocenoid D	C21H28O10	Iridoid (bis-iridoid)	Root	20
10	Pterocenoid E	C23H32O9	Iridoid (bis-iridoid)	Root	22
11	Hookerinoid A	C23H32O9	Iridoid (bis-iridoid)	Root	24
12	Laciniatoside I	C28H38O14	Iridoid (bis-iridoid)	Root, whole plant	20, 25–28
13	Pteroceside D	C36H56O15	Iridoid (bis-iridoid)	Root, whole plant	22 and 25
14	Triplostoside A	C35H52O20	Iridoid (bis-iridoid)	Root, aerial regions, whole plant	16–20, 22, 29
15	Cantleyoside	C33H46O19	Iridoid (bis-iridoid)	Root, aerial regions, whole plant	15–18, 20, 22, and 27
16	Sylvestroside I	C33H48O19	Iridoid (bis-iridoid)	Root, whole plant	16, 19, 20, 22, 27, 29
17	Sylvestroside III dimethyl acetal	C29H42O15	Iridoid (bis-iridoid)	Root, whole plant	22 and 27
18	Sylvestroside III	C27H36O14	Iridoid (bis-iridoid)	Root, whole plant	20, 22, 27, 28
19	Hookerinoid B	C23H34O11	Iridoid (bis-iridoid)	Root	25
20	Pterocenoid H	C23H34O11	Iridoid (bis-iridoid)	Root	22 and 25
21	Pteroceside A	C35H54O15	Iridoid (bis-iridoid)	Root, whole plant	22 and 25
22	Pteroceside B	C33H50O15	Iridoid (bis-iridoid)	Root, whole plant	22 and 25
23	Pteroceside C	C32H48O15	Iridoid (bis-iridoid)	Root, whole plant	22 and 25
24	Laciniatoside II	C25H34O12	Iridoid (bis-iridoid)	Root, whole plant	20,22,26–28
25	Sylvestroside IV	C27H36O14	Iridoid (bis-iridoid)	Root, whole plant	22,27,28
26	Sylvestroside IV dimethyl acetal	C29H42O15	Iridoid (bis-iridoid)	Root, whole plant	19,22 and 29
27	Pterocenoid A	C21H23NO7	Iridoid (bis-iridoid)	Root	23
28	Pterhookeroside	C21H34O10	Iridoid (bis-iridoid)	Root	21
29	5-[3-(1-Hydroxyethyl) pyridine], 7-loganin ester	C25H33NO12	Iridoid (bis-iridoid)	—	16
30	Dipsanoside A	C66H88O38	Iridoid (oligomer)	Aerial regions, whole plant	16 and 17
31	Pterocephanoside	C57H74O30	Iridoid (oligomer)	Aerial regions	17
32	Dipsanoside B	C66H90O37	Iridoid (oligomer)	Aerial regions, whole plant	17
33	Pterocephanoside A	C58H78O30	Iridoid (oligomer)	Whole plant	30
34	Chlorogenic acid	C16H18O9	Phenylpropanoid (simple phenylpropanoid)	Whole plant	18, 20 and 31
35	3,4-Dicaffeoylquinic acid	C25H24O12	Phenylpropanoid (simple phenylpropanoid)	Whole plant	20
36	3,5-Dicaffeoylquinic acid	C25H24O12	Phenylpropanoid (simple phenylpropanoid)	Whole plant	20
37	4,5-Dicaffeoylquinic acid	C25H24O12	Phenylpropanoid (simple phenylpropanoid)	Whole plant	20
38	Isochlorogenic acid A	C25H24O12	Phenylpropanoid (simple phenylpropanoid)	Whole plant	18
39	Isochlorogenic acid C	C25H24O12	Phenylpropanoid (simple phenylpropanoid)	Whole plant	18
40	2,5-Dihydroxycinnamic acid methyl ester	C10H10O4	Phenylpropanoid (simple phenylpropanoid)	Whole plant	19
41	3,4-Dihydroxycinnamic acid methyl ester	C10H10O4	Phenylpropanoid (simple phenylpropanoid)	Whole plant	29
42	(+)-1-Hydroxypinoresinol 4′-O-β-D-glucopyranoside	C26H32O12	Phenylpropanoid (lignan)	Whole plant	21 and 29
43	(+)-1-Hydroxypinoresinol 4′′-O-β-D-glucopyranoside	C26H32O12	Phenylpropanoid (lignan)	Whole plant	21 and 29
44	(+)-Syringaresinol-β-D-glucoside	C28H36O13	Phenylpropanoid (lignan)	Root	21
45	8-Hydroxypinoresionol-4′-O-β-D-glucoside	C26H32O14	Phenylpropanoid (lignan)	Whole plant	32
46	8′-Hydroxylpinoresionol-4′-O-β-D-glucoside	C26H32O14	Phenylpropanoid (lignan)	Whole plant	32
47	(7R,8S)-Erythro-7,9,9′- trihydroxy-3,3′-dimethoxy-8-O-4′-neolignan-4-O-β-D-glucoside	C26H36O12	Phenylpropanoid (lignan)	Root	21
48	Cedrusin-4-O-β-glucoside	C26H34O11	Phenylpropanoid (lignan)	Root	21
49	Ptehoosine A	C31H32O9	Phenylpropanoid (lignan)	Whole plant	33
50	Ptehoosine B	C31H32O9	Phenylpropanoid (lignan)	Whole plant	33
51	Syringaresinol	C22H26O8	Phenylpropanoid (lignan)	Whole plant	33
52	Pinoresinol	C20H22O6	Phenylpropanoid (lignan)	Whole plant	33
53	Hookeroside A	C52H84O20	Triterpenoid (oleanane-type)	Whole plant	6, 19, 20 and 29
54	Hookeroside B	C57H92O24	Triterpenoid (oleanane-type)	Whole plant	6, 19, 20 and 29
55	Hookeroside C	C83H102O28	Triterpenoid (oleanane-type)	Whole plant	6, 19, 20 and 29
56	Hookeroside D	C71H82O18	Triterpenoid (oleanane-type)	Root, whole plant	6, 15, 19, 20 and 29
57	Songoroside A	C35H56O7	Triterpenoid (oleanane-type)	Whole plant	6, 15, 19, 20 and 29
58	Oleanolic acid	C30H48O3	Triterpenoid (oleanane-type)	Whole plant	6, 15, 19, 20 and 29
59	Rivularicin	C62H100O27	Triterpenoid (oleanane-type)	Whole plant	19, 20 and 34
60	Oleanolic acid 3-O-β-D-xylopyranosyl(1→3)-α-L-rhammopyranosyl(1→2)-β-D-xylopyranoside	C43H56O19	Triterpenoid (oleanane-type)	Whole plant	19 and 29
61	Oleanolic acid 3-O-β-D-xylopyranosyl(1→4)-β-D-glucopyranosyl (1→2)-β-D-xylopyranoside	C47H74O30	Triterpenoid (oleanane-type)	Whole plant	19 and 29
62	Oleanolic acid 3-O-β-D-glucopyranosyl(1→4)-β-D-xylopyranosy(1→3)-α-L-rhamnopyanosyl(1→2)-β-D-xylopyranoside	C42H46O25	Triterpenoid (oleanane-type)	Whole plant	19 and 29
63	Giganteaside D	C31H48O15	Triterpenoid (oleanane-type)	Whole plant	19, 20 and 29
64	Prosapogenin Ax	C42H68O24	Triterpenoid (oleanane-type)	Whole plant	6 and 29
65	Prosapogenin Bx	C47H75O30	Triterpenoid (oleanane-type)	Whole plant	6 and 29
66	Pterocephin A	C52H84O35	Triterpenoid (oleanane-type)	Whole plant	35
67	Oleanonic acid	C30H46O3	Triterpenoid (oleanane-type)	Whole plant	36
68	Ursolic acid	C30H48O3	Triterpenoid (ursane-type)	Whole plant	6, 15, 19 and 20
69	11,12-Epoxy-2,6-dihydroxy-24-norursa-1,4-dien-3-on-2-on-(28→13)-olide	C28H36O6	Triterpenoid (ursane-type)	Root	24
70	Hookerinoid C	C28H40O5	Triterpenoid (ursane-type)	Root	24
71	Palmitic acid	C16H32O2	Fatty acid (saturated)	Whole plant	6 and 15
72	Heptadecanoic acid	C17H34O2	Fatty acid (saturated)	Whole plant	37
73	Pentadecanoic acid	C15H30O2	Fatty acid (saturated)	Whole plant	37
74	Myristic acid	C14H28O2	Fatty acid (saturated)	Whole plant	37
75	Stearic acid	C18H36O2	Fatty acid (saturated)	Whole plant	37
76	Arachidic acid	C20H40O2	Fatty acid (saturated)	Whole plant	37
77	Behenic acid	C22H44O2	Fatty acid (saturated)	Whole plant	37
78	Lignoceric acid	C24H48O2	Fatty acid (saturated)	Whole plant	37
79	Lignoceric acid	C23H46O2	Fatty acid (saturated)	Whole plant	37
80	Ginkgolic acid	C22H34O3	Fatty acid (unsaturated)	Whole plant	37
81	Linoleic acid	C18H32O2	Fatty acid (unsaturated)	Whole plant	37
82	Oleic acid	C18H34O2	Fatty acid (unsaturated)	Whole plant	37
83	Palmitoleic acid	C16H30O2	Fatty acid (unsaturated)	Whole plant	37
84	Eicosenoic acid	C20H38O2	Fatty acid (unsaturated)	Whole plant	37
85	α-Linolenic acid	C18H30O2	Fatty acid (unsaturated)	Whole plant	37
86	5,7,3′,4′,6′-Pentahydroxyflavanone	C15H12O7	Flavonoid (flavanone)	Whole plant	36
87	Luteolin	C15H10O6	Flavonoid (flavone)	Whole plant	8
88	L-Methionine	C5H11NO2S	Amino acid	Whole plant	36
89	p-Hydroxy benzaldehyde	C7H6O2	Phenolic acid	Whole plant	36
90	β-Gentiobiose	C12H22O11	Carbohydrate	Whole plant	15
91	Twenty alkyl ethers	C40H82O	Ethers	Whole plant	36
92	Pentatriacontane	C35H72	Alkane	Whole plant	36
93	β-Sitosterol	C29H50O	Steroid	Whole plant	36

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4.1 Iridoids

Iridoids are monoterpenoids composed of six-membered oxygen heterocycles and fused cyclo-pentane rings. Iridoids are usually subdivided into four groups: iridoid glycosides, bis-iridoids, seco-iridoids, and non-glycosidic iridoids.^38–40 The main compounds isolated from the different parts of P. hookeri are iridoids, and 33 iridoids (Table 2) have been isolated. According to their structures, they were divided into four groups, compounds 1–4 (four iridoids), compounds 5, 6 (two secoiridoids), compounds 7–29 (23 bis-iridoids), and compounds 30–33 (four iridoid oligomers) iridoid glycosides. The structures of these compounds are shown in Fig. 3.

Fig. 3 The structures of the iridoids obtained from P. hookeri.

The earliest reports on iridoids in P. hookeri were published in 2000. Tian et al. separated loganin (compound 1) from the whole plant of P. hookeri for the first time, and the compound was considered the predominant compound.¹⁵ Wu et al. reported 24 iridoids (compounds 4, 5, 7–9, 10–28). Compounds 7–11, 19–20, 27 were originally isolated from P. hookeri. The bis-iridoids may be the key ingredients which account for the anti-inflammatory effects of P. hookeri, and compounds 7, 8, 27 can inhibit TNF-α-induced NF-κB-dependent promoter activity.^22–25 Furthermore, Zhang et al. isolated three iridoid glycosides from the 95% ethanol extracts from the whole plant of P. hookeri in 2014, namely, loganetin (compound 2), 5-[3-(1-hydroxyethyl) pyridine], 7-loganin ester (compound 29), and dipsanoside A (compound 30), using chemical methods and spectral analysis.¹⁶ In addition, Huang et al. isolated two iridoid glycosides (compounds 31, 32) from the 95% ethanol extracts of the aerial parts of P. hookeri. Compound 32 is a novel iridoid oligomer. Huang et al. elucidated its structure using extensive spectroscopic analysis, including 1D-NMR and 2D-NMR experiments, and showed that it had no significant activity against MCF-7 (human breast cancer), HEPG2 (human liver carcinoma), and H460 (human large cell cancer of the lung) cancer cells (IC₅₀ > 50 μM, n = 3).¹⁷ Moreover, Tang et al. detected two iridoid glycosides (compounds 3, 6) from 70% methanol extracts of P. hookeri using UPLC-Q-TOF/MS and identified them by using standard products.²⁰ Most recently, pterocephanoside A (compound 33) was isolated from P. hookeri, and the compound mostly possessed seco-iridoid subtype and iridoid subtype skeletons and showed an inhibitory effect on LPS-induced NO production in RAW 264.7 cells (murine macrophages), but the activity was weak.³⁰

4.2 Phenylpropanoids

The skeleton of phenylpropanoid is formed from C6–C3 units, however, some of the carbons in the side chain might have been lost during the biosynthesis. Phenylpropanoids are widely found in traditional Chinese medicine and have various physiological activities, such as antioxidation, anti-inflammatory, antibacterial, hemostasis, antitumor, and they can give cardiovascular protection.^41–43 To date, 19 phenylpropanoids have been detected this plant, and they are divided into two groups: simple phenylpropanoids (compounds 34–41) and lignans (compounds 42–52), according to their structures, and they are shown in Fig. 4.

Fig. 4 The skeletal structures of phenylpropanoids obtained from P. hookeri.

Compounds (42–44, 47, 48) were isolated and purified from P. hookeri with silica gel chromatography using a Sephadex LH-20 column, semipreparative HPLC, NMR, and HR-ESIMS. It should be noted, that these compounds have never been found in the genus Pterocephalus before, and have the potential to become useful chemotaxonomic markers of Pterocephalus.²¹ Li et al. established a UFLC-PDA fingerprint analysis method for use with P. hookeri from different producing areas, and identified five components with standards, including compound 34.³¹ Two phenylpropanoids (compounds 45, 46) were separated and identified in the P. hookeri 95% ethanol extracts by spectral analyses, including HR-ESIMS, NMR, and HPLC.³² Compound 40 and 41 were originally isolated from P. hookeri.^19,29 Tang et al. using a standard comparison method, identified three phenylpropyl compounds (compounds 35–37) from a 70% methanol extract of P. hookeri using UPLC-Q-TOF/MS.²⁰ Meanwhile, Wang et al. used UPLC-PDA to identify compounds 38, 39 from a methanol extract of P. hookeri, and proved that these compounds could be absorbed by rats using the everted intestinal sac model.¹⁸ Two undescribed lignans (compounds 49, 50) and two known lignans (compounds 51, 52) were isolated by Dong et al.³³ Compound 49 was found to be effective in inhibiting angiogenesis.³²

4.3 Triterpenoids

Terpenoids represent the largest and most diverse class of natural products produced by plants.⁴⁴ Terpenoids are abundant in P. hookeri and play an important role in many important physiological activities and biological functions. The main terpenoids in P. hookeri are two types of triterpenoids, including the oleanane type (compounds 53–67) and the ursane type (compounds 68–70). A total of 18 triterpenoids have been reported, and most of them share the same skeleton as the oleanane type. One of the characteristics of the oleanane type pentacyclic triterpenoids of P. hookeri is their aglycones, which are always replaced by glycosyl groups at the 3 or 28 sites. Their structures are shown in Fig. 5.

Fig. 5 The structures of triterpenoids obtained from P. hookeri.

Using DA 201 resin, silica gel, and spectroscopic analysis methods, such as IR and NMR, Tian et al. separated and identified seven triterpenoids (compounds 53–58, 68) from the 95% ethanol extracts of the whole plant of P. hookeri. This study was the first to isolate these compounds from P. hookeri.⁶ In 2002, Zhang et al. isolated rivularicin (compound 59) from P. hookeri for the first time.³⁴ They also separated oleanonic acid (compound 67) from the 95% ethanol extracts of the whole plant of P. hookeri, and then established a quality control method for P. hookeri analysis using the oleanolic acid content. In addition, they verified that oleanonic acid exerts inhibitory effects on Staphylococcus aureus, Escherichia coli, and Bacillus subtilis.³⁶ Another two triterpenoids (compounds 69, 70) were isolated and identified, and compound 69 was found to significantly inhibit the proliferation of Hep3B cells (human hepatocyte carcinoma), with an IC₅₀ of 17.06 μm.²² Yu et al. isolated and purified four triterpenoids (compounds 60–63) with silica gel and Sephadex LH-20 columns and then determined their spectral data.¹⁹ Zhang et al. studied the chemical components in the 90% ethanol extracts of P. hookeri using physical and chemical properties obtained by spectral data analysis and identified another two triterpenoids (compounds 64, 65).²⁹ Wang et al. isolated pterocephin A (compound 66) from P. hookeri. According to published pharmacological studies, the survival rates of L-02 cells (human fetal hepatocytes) decreased significantly when treated with pterocephin A at a concentration of 16 μmol L⁻¹, showing that the compound has obvious cytotoxicity.³⁵

4.4 Fatty acids

Fatty acids, used to be considered as only energy sources and structural components of the cell membrane, but they now show great potential for the treatment of several diseases, such as type II diabetes mellitus, nonalcoholic steatohepatitis, and chemically induced liver injury.^45–47 A total of 15 fatty acids have been isolated from P. hookeri, and these were divided into two group: saturated fatty acids (compounds 71–79) and unsaturated fatty acid (compounds 79–85), and their structures are shown in Fig. 6.

Fig. 6 The structures of fatty acids obtained from P. hookeri.

Palmitic acid (compound 71) was the first reported fatty acid found in P. hookeri.¹⁵ Then, by using GC-MS, Zhang et al. detected 14 fatty acid components (compounds 72–85) from P. hookeri at the flowering and nonflowering stages. The compounds were identified by comparison with standards. Moreover, α-linolenic acid, a plant-derived n-3 polyunsaturated fatty acid, is a potential fatty acid biomarker because it is useful in distinguishing between the two groups.^36,37

4.5 Other compounds

Some compounds do not belong to the previous classifications, such as flavonoids, carbohydrates, and steroids. Their structures are shown in Fig. 7.

Fig. 7 The structures of compounds 86–93 obtained from P. hookeri.

In 2013, Zhang et al. isolated six compounds (compounds 86, 88, 89, 91–93) from a 95% ethanol extract of a whole P. hookeri plant and identified them with chemical methods and spectral analysis. Compound 86 was found to have a significant inhibitory effect on Staphylococcus aureus.³⁶ The only carbohydrate isolated from P. hookeri is β-gentiobiose (compound 90).¹⁵ In addition to compound 86, another flavonoid, luteolin (compound 87), was separated from the whole plant of P. hookeri.²⁹ It is a common ingredient with a wide range of pharmacological activities, such as antitumor, lipid lowering, anti-inflammatory and antioxidant activities.^48–50

5. Pharmacological activities

P. hookeri is in widespread use, and its extracts and chemical components have been used in in vitro and in vivo experimental models. All its pharmacological activities are summarized in Fig. 8 and the following subsections will describe these in more detail.

Fig. 8 Overview of the pharmacological activities of P. hookeri.

5.1 Anti-inflammatory activity

The primary anti-inflammatory evaluation of P. hookeri was based mainly on the mouse models of xylene-induced ear edema and acetic acid-induced peritoneal capillary permeability, and rat models of carrageenan/fresh egg white-induced paw edema and cotton/agar pellet granuloma. In 2004, Guan et al. evaluated the anti-inflammatory activity of an n-butanol extract of P. hookeri, and the results showed that the extract had significant inhibitory effects on xylene-induced ear edema in mice and fresh egg white-induced paw edema in rats (P < 0.05) when the dose was 0.25–0.7 g kg⁻¹.⁵¹ Interestingly, Zhang et al. (2009), Shen et al. (2017), and Chen et al. (2018) reported similar results.^27,52,53 Another model of acute inflammation is acetic acid-induced peritoneal capillary permeability. Acetic acid can increase the levels of prostaglandins, histamines, 5-hydroxytryptamine and other chemical mediators in the abdominal fluid, thus increasing vascular permeability. Using an ethanol extract of P. hookeri (2 g kg⁻¹), water extract (4 g kg⁻¹), and total glycosides (28–112 mg kg⁻¹, the main components found are compounds 1, 5, 15, 16) which can significantly inhibit the increase in vascular permeability induced by acetic acid in mice. The cotton/agar pellet granuloma model and dry weight of chronic inflammation correlated with the level of granulomatous tissue formation. An ethanol extract of P. hookeri (2 g kg⁻¹), n-butanol extract (0.25–0.5 g kg⁻¹), and total glycosides (28–112 mg kg⁻¹) could effectively inhibit cotton ball granuloma. Therefore, in addition to the acute stage of inflammation, the extracts of P. hookeri showed anti-inflammatory activity in the chronic stage of inflammation as well.^52,53 Bis-iridoids from P. hookeri (25 and 50 μM, the main components were compounds 11 and 19) significantly reduced TNF-α- and LPS-induced NF-κB activation in HEK293 cells (human embryonic kidney), and the main components, compounds 12, 15–18, 24–26, reduced the production of inflammatory cytokines in a dose-dependent manner.^24,27

5.2 Anti-rheumatoid arthritis

Rheumatoid arthritis (RA) is chronic auto-immune disorder, its main characteristics are swelling and pain in joints, and in synovial membranes. The main treatment is to suppress inflammation and relieve pain.⁵⁴ In Tibetan medicine, RA is known as “zhen bu” disease, and the clinical efficacy of Tibetan medicine on RA is as high as 94.6%.⁵⁵ P. hookeri is commonly used as a treatment for RA in Tibet. The animal adjuvant arthritis (AA) model is a contraindicated animal model mediated by T-cell immunity.^56–59 It is a commonly used animal model of RA in clinics because of its simplicity in modeling and consistency with clinical manifestations, pathomorphology, and immunological changes in patients with RA.⁶⁰ This model has been adopted in studies on the anti-arthritis effects of P. hookeri. Shen et al.⁵³ and Yang et al.⁵⁶ investigated the efficacy and mechanism of using the total glucosides of P. hookeri on AA rats and found that the total glycosides of P. hookeri (28, 56, 116, and 232 mg kg⁻¹) significantly reduced primary paw swelling, secondary paw swelling, and arthritis scores in the later stages of AA. In addition, the total glucosides of P. hookeri not only improved the proliferation of synovial cells, macrophages, and microcells, and induced inflammatory cell infiltration but it also significantly reduced the levels of IL-1β, TNF-α, and IL-17. However, these effects were not dose-dependent. Notably, the total glycosides of P. hookeri not only significantly increased MDA and NO levels but also significantly decreased the SOD level and the expression of NF-κB p65 in the synovial tissues of the joints. This result suggested that the anti-RA effect of total glycosides of P. hookeri may be related to the antioxidant effect and the inhibition of the NF-κB signaling pathway.^53,56

5.3 Analgesic activity

The analgesic effects of P. hookeri were studied using several types of pain models, including acetic acid-induced abdominal writhing reflex (peripheral pain) and pain caused by a hot plate in mice (central pain). In 2017, the analgesic abilities of total glycosides from P. hookeri were evaluated by Shen et al.⁵³ At doses of 56 and 112 mg kg⁻¹, obvious analgesic effects on pain induced by acetic acid were observed. However, in a hot-plate test, the pain threshold only increased 30 min after treatment with 112 mg kg⁻¹ dose. The total glycosides from P. hookeri showed a good peripheral analgesic effect, but the central analgesic effect was not obvious. The peripheral analgesic effect may be related to P. hookeri's good anti-inflammatory effect.⁵³ Moreover, Chen et al.²⁷ showed that the bis-iridoid constituents from P. hookeri (50 and 100 mg kg⁻¹) significantly increased the hot-plate pain threshold and reduced the acetic acid-induced writhing response in mice (P < 0.01), and the efficacy of high-dose bis-iridoid constituents from P. hookeri (100 mg kg⁻¹) was superior to that of the positive control (rotundine, 20 mg kg⁻¹). The results are similar to those of Zhang's⁵² previous experiment. These results indicated that iridoids possess analgesic effects.^27,52

5.4 Anti-cancer activity

Cancer is not only a serious threat to people's health but also a difficult and hot topic of research. P. hookeri has antitumor effects. The total saponins of P. hookeri have inhibitory effects on a variety of cancer cells, such as SGC-7901 (human gastric cancer cells), HepG2, AGS gastric adenocarcinoma cells from a human stomach, and MBA-MD-231 (human breast cancer cells).⁶¹ In 2015, Guo et al. conducted an in-depth study on the antitumor effect of an n-butanol extract of P. hookeri (the main components were compounds 15, 16, 18, 24, 25) on Hep3B cancer cells and found that the extract can selectively inhibit the proliferation of Hep3B cells in vitro, induce apoptosis, block the PI3K pathway, and regulate the protein level of the Bcl-2 family. In addition, the extract inhibited tumor growth by regulating the expression of Bcl-2 family proteins in xenografted tumor mouse models.^28,62

5.5 Neuroprotection

Parkinson's disease, also known as ‘wobbly paralysis’ in China, is a chronic neurodegenerative disease caused by extrapyramidal dysfunction. The disease usually occurs in middle age or later. The main cause of Parkinson's disease is the damage of dopaminergic neurons in the substantia nigra of the brain, and a decrease in dopamine biosynthesis in the striatum, which results in a significant reduction in dopamine transmitters and hypercholinergic nerve function, and these result in movement disorders. The neuroprotective effects of a n-butanol extract of P. hookeri (the main components are compounds 13–18, 24, 25) have been demonstrated in transgenic zebrafish models. The DAT-GFP fertilized eggs treated with 100 μg mL⁻¹ n-butanol extract were able to resist damage from to H₂O₂, and the number of dopamine neurons in the fertilized eggs was the same as that in the control group.²⁶

6. Toxicology

Although P. hookeri has been used as a traditional Tibetan medicine for a long time, its potential toxicity has not been systematically elucidated. According to the records from the classical ancient books, P. hookeri has always been considered as a drug with a low toxicity, but reports about its toxicity are few. In early toxicity tests, mice received an oral water extract of P. hookeri 3000 times the clinical dose. The mice ate less and showed considerable weight loss. These results indicated that P. hookeri had some level of toxicity, but the study was not thorough.⁵¹

In view of the previous research results, Wang et al. evaluated the hepatotoxicity of an n-butanol extract of P. hookeri in 2019 and found that the serum levels of ALP, ALT, AST, DBIL, and TBIL were significantly increased (P < 0.05, P < 0.01) in the mice in the group which had received an n-butanol extract of P. hookeri. The results showed that the extract caused a certain degree of liver damage in the mice. The expression levels of NF-κB, RIP1, and RIP3 in the liver tissues and L-02 cells were upregulated after treatment with the extract and the L-02 hepatocytes. These results indicated that the extract can induce liver toxicity in vivo and in vitro, which can induce the development of inflammation and subsequent necrosis.⁶³

7. Qualitative and quantitative analysis

The most recent quality control standard for P. hookeri is in the 2020 edition of the Chinese Pharmacopoeia, in which microscopic and TLC identification is mainly used for qualitative analysis, and quantitative analysis mainly uses HPLC.¹⁰ Before 2013, a large number of studies showed that P. hookeri mainly contains triterpenoid saponins, and oleanolic acid and ursolic acid were considered to be the main quality control components of P. hookeri. Therefore, a variety of methods for the determination of oleanolic acid and ursolic acid were established, such as HPLC-evaporative light-scattering detection, HPLC with a photodiode array, and capillary zone electophoresis.^37,64–66 In 2018, a UPLC-Q-TOF/MS method was established for the analysis of the chemical constituents of P. hookeri. A total of 17 iridoid glycosides, 7 phenolic acids, 13 triterpenes, and 3 other components were identified or preliminarily deduced. The 10 main components found were dipsanoside B, sweroside, cantleyoside, chlorogenic acid, loganic acid, loganin, sylvestroside I, dipsanoside A, isochlorogenic acid A, and isochlorogenic acid C, which were quantified using UPLC-PDA. Notably, iridoid glycosides and phenolic acids were found to be the main active components of the 40 compounds identified using molecular docking. The current quality control standard of P. hookeri uses oleanolic acid and ursolic acid as quality control indices, which cannot effectively control the quality of the medicinal material, lack direct correlation with biological activity, and are insufficient in evaluating the quality of P. hookeri. Therefore, a more suitable and feasible method is needed to comprehensively evaluate the quality of P. hookeri.^20,67

8. Conclusion and perspectives

P. hookeri is not only a typical plateau herbaceous plant but also one of the commonly used Tibetan medicines for clearing heat, stopping dysentery, detoxification, eliminating distemper, dispelling wind, and relieving stagnation. This paper reviews the progress of research on P. hookeri from the aspects of traditional use, phytochemistry, pharmacology, and toxicology. Because of limited resources and investment, the study of P. hookeri and its decoctions have not received enough attention, and the study of its pharmacological activities and related molecular mechanisms is insufficient.

The chemical structures of the compounds found in P. hookeri plants are structurally diverse, and mainly include triterpenes, iridoid glycosides, phenolic acids, flavonoids, and other compounds. The crude extract and chemical components of monomers isolated from the plants are still in the preliminary stage, but they do show certain anti-inflammatory, antitumor, neuroprotective, and anti-rheumatoid arthritis activities. However, the identity of the pharmacodynamic substance is still unclear. Modern phytochemical and pharmacological studies have provided some evidence of some of the mechanisms of the action of P. hookeri and demonstrate its further development potential as an anti-inflammatory and antitumor agent.

In addition, quality markers from P. hookeri are necessary for the study of the mechanisms of action and quality control of medicinal materials. Currently, only oleanolic acid and ursolic acid have been selected as the quality indicators of P. hookeri in the Chinese Pharmacopoeia in 2020. However, these compounds cannot effectively control the quality of P. hookeri. Therefore, chemicals and biomarkers that can better reflect the quality of P. hookeri are needed.

In conclusion, long-term clinical practice has proven the safety and efficacy of P. hookeri. To fully explore the medicinal value of P. hookeri, modern advanced research techniques (mass spectroscopy imaging technology and patch clamp technology) should be used to systematically study its absorption, distribution, metabolism, excretion, tissue distribution, mechanism of action, and quality evaluation, for the development of the use of P. hookeri.

Abbreviations

TNF-α	Tumor necrosis factor-α
NF-κB	Nuclear factor kappa-B
NMR	Nuclear magnetic resonance
UPLC-Q-TOF/MS	Ultra-performance liquid chromatography coupled with time-of-flight mass spectrometry
LPS	Lipopolysaccharide
NO	Nitric oxide
HPLC	High-performance liquid chromatography
HR-ESIMS	High resolution-electrospray ionization mass spectrometry
UFLC-PDA	Ultra-flow liquid chromatography-photo diode array
IR	Infrared spectroscopy
IC50	50% inhibition concentration
GC-MS	Gas chromatography-mass spectrometry
RA	Rheumatoid arthritis
AA	Adjuvant arthritis
IL-1β	Interleukin-1β
IL-17	Interleukin-17
MDA	Malonaldehyde
SOD	Superoxide dismutase
Bcl-2	B-cell lymphocytoma-2
ALT	Alanine aminotransferase
AST	Aspartate aminotransferase
ALP	Alkaline phosphatase
DBIL	Direct bilirubin
TBIL	Total bilirubin
TLC	Thin layer chromatography
Glc	Glucose
Xyl	Xylose
Rha	Rhamnose
Api	Apidside

Author contributions

Zhiqiang Gan – resources, supervision, visualization, writing – original draft, writing – review and editing. Juan Jiang – writing – original draft, writing – review and editing. Honglin Tao – resources, writing – original draft. Shiying Luo – resources. Xianli Meng – supervision. Jia Yu – supervision, writing – review and editing, funding acquisition. Yi Zhang – supervision, funding acquisition. Ce Tang – resources, supervision, writing – original draft, writing – review and editing, funding acquisition.

Conflicts of interest

The authors declare no conflict of interest.

Acknowledgements

The authors gratefully acknowledge the financial support from the National Natural Science Foundation of China (No. 81903922 and 81803851), the National Key Research and Development Program of China (No. 2017YFC1703900), and the “Xinglin Scholars” Research Promotion Program of Chengdu University of Traditional Chinese Medicine (BSH2019002).

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Footnote

† These authors contributed equally to this work.

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