Issue 2, 2021

Short oligoalanine helical peptides for supramolecular nanopore assembly and protein cytosolic delivery

Abstract

In this work we report a rational design strategy for the identification of new peptide prototypes for the non-disruptive supramolecular permeation of membranes and the transport of different macromolecular giant cargos. The approach targets a maximal enhancement of helicity in the presence of membranes with sequences bearing the minimal number of cationic and hydrophobic moieties. The here reported folding enhancement in membranes allowed the selective non-lytic translocation of different macromolecular cargos including giant proteins. The transport of different high molecular weight polymers and functional proteins was demonstrated in vesicles and in cells with excellent efficiency and optimal viability. As a proof of concept, functional monoclonal antibodies were transported for the first time into different cell lines and cornea tissues by exploiting the helical control of a short peptide sequence. This work introduces a rational design strategy that can be employed to minimize the number of charges and hydrophobic residues of short peptide carriers to achieve non-destructive transient membrane permeation and transport of different macromolecules.

Graphical abstract: Short oligoalanine helical peptides for supramolecular nanopore assembly and protein cytosolic delivery

Supplementary files

Article information

Article type
Paper
Submitted
19 6 2020
Accepted
07 11 2020
First published
08 12 2020
This article is Open Access
Creative Commons BY-NC license

RSC Chem. Biol., 2021,2, 503-512

Short oligoalanine helical peptides for supramolecular nanopore assembly and protein cytosolic delivery

M. Pazo, G. Salluce, I. Lostalé-Seijo, M. Juanes, F. Gonzalez, R. Garcia-Fandiño and J. Montenegro, RSC Chem. Biol., 2021, 2, 503 DOI: 10.1039/D0CB00103A

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