Feature Article
CRISPR–Cas based platforms for RNA detection: fundamentals and applications
CRISPR-Cas technologies are turning into new RNA detection platforms for diagnosing many urgent diseases such as infections and cancer.
Enzyme inhibition-enabled CRISPR/Cas12a biosensing system for heparin-related non-nucleic acid biomarkers
An enzyme inhibition-enabled CRISPR/Cas12a biosensing strategy is proposed for detecting heparin-related non-nucleic acid biomarkers.
CRISPR-Cas13a-based dual-channel AND-logic gated biosensor for the simultaneous assay of APE1 and miRNA-224
Simultaneous detection of multiple targets is of great significance for the precise diagnosis of diseases.
A novel CRISPR–Cas12a-based fluorescence anisotropy method with a high signal-to-background ratio for sensitive biosensing
Herein, a novel CRISPR–Cas12a-based fluorescence anisotropy method with a high signal-to-background ratio was designed for sensitive biosensing.
A multi-functional synergistic platform of Cas12a split dsDNA activators
This work comprehensively explores the effects of various positions of Cas12a split activators.
Catalytic hairpin assembly-coupled CRISPR/Cas12a biosensor for sensitive detection of melamine in dairy products
We developed a biosensor integrating catalytic hairpin assembly and Cas12a for highly sensitive and specific melamine detection in dairy products.
Unveiling a novel RNA G-triplex structure: its function and potential in CRISPR-based diagnostics
The truncated TERRA sequence [UUAGGG]3 forms a stable parallel RNA G-triplex structure and can be used as a novel biosensing element.
Pyrococcus furiosus Argonaute-mediated dual recognition enables the detection of trace single-nucleotide-mutated fungicide-resistant fungal pathogens
We developed an assay, termed PARPA for sensitive detection of trace single-nucleotide mutations in genomes of fungicide-resistant pathogens.
About this collection
Although best known as gene editing tools, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated (Cas) systems have also emerged as one of the most advanced classes of molecular machineries that offer diverse biological functions suitable for biosensor development. With their remarkable sensitivity, specificity, and simplicity, CRISPR-based biosensors are highly attractive to address challenges in medical diagnosis, molecular imaging, food safety, environmental surveillance, and beyond. To achieve the goal of real-life applications, it is critical to further engineer CRISPR Cas machineries and design advanced sensing systems to match the ongoing needs.
Guest edited by Professor Feng Li (Sichuan University, China), Professor Souvik Maiti (CSIR-Institute of Genomics and Integrative Biology, India) and Professor Mingqiang Li (Sun Yat-sen University, China), this collection aims to feature research reporting new designs and applications of CRISPR systems for biosensing.