CRISPR-Cas13a-based dual-channel AND-logic gated biosensor for the simultaneous assay of APE1 and miRNA-224

Tongyan Yuan; Jinlian Du; Jingjing Hu; Jiaming Luo; Xingxiang Pu; Xinyue Zhu; Bojie Chen; Yuling Teng; Han Li; Sheng Li; Ling Jiang; Erhu Xiong

doi:10.1039/D5CC03222A

CRISPR-Cas13a-based dual-channel AND-logic gated biosensor for the simultaneous assay of APE1 and miRNA-224†

Tongyan Yuan,^a Jinlian Du,^a Jingjing Hu,^a Jiaming Luo,^a Xingxiang Pu,*^b Xinyue Zhu,^a Bojie Chen,^a Yuling Teng,^a Han Li,^a Sheng Li,^a Ling Jiang

*^a and Erhu Xiong

*^a

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* Corresponding authors

^a Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China
E-mail: ljiang630@163.com, xiongerhu2008@163.com

^b Department of Medical Oncology, Lung Cancer and Gastrointestinal Unit, Hunan Cancer Hospital & The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410013, China
E-mail: puxingxiang@hnca.org.cn

Abstract

Simultaneous detection of multiple targets is of great significance for the precise diagnosis of diseases. Herein, we report a dual-channel AND-logic gated biosensing platform based on the CRISPR-Cas13a system for the simultaneous detection of APE1 and miRNA-224, which showed superior specificity, sensitivity and potential for practical applications. Our study not only expands the CRISPR toolbox beyond nucleic acid assay but also establishes a new paradigm for multi-analyte diagnostic systems.

This article is part of the themed collection: CRISPR for Biosensing

Chemical Communications

CRISPR-Cas13a-based dual-channel AND-logic gated biosensor for the simultaneous assay of APE1 and miRNA-224†

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CRISPR-Cas13a-based dual-channel AND-logic gated biosensor for the simultaneous assay of APE1 and miRNA-224

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