Issue 5, 2015

Fluorescent silver nanocluster DNA probes for multiplexed detection using microfluidic capillary electrophoresis

Abstract

DNA-stabilized fluorescent silver nanoclusters (AgNC DNA) are a new class of fluorophore that are formed by sequence specific interactions between silver and single-stranded DNA. By incorporating both target-binding and fluorescent-reporting sequences into a single synthetic DNA oligomer, AgNC DNA probes eliminate the need to conjugate dye or quencher molecules. In this study, we modify a AgNC DNA probe to demonstrate single-color multiplexed detection of DNA targets. We show that appending different lengths of poly-dT to the probe sequences tunes the electrophoretic mobility of AgNC DNA probes without affecting their fluorescence spectra. We use this to introduce a set of AgNC DNA probes selective for Hepatitis A, B and C target sequences that can be processed together in a simple, single-step protocol and distinguished with a resolution of 3.47 and signal to noise ratio of 17.23 in under 10 seconds by microfluidic capillary electrophoresis.

Graphical abstract: Fluorescent silver nanocluster DNA probes for multiplexed detection using microfluidic capillary electrophoresis

Supplementary files

Article information

Article type
Paper
Submitted
24 sep. 2014
Accepted
19 des. 2014
First published
09 jan. 2015

Analyst, 2015,140, 1609-1615

Author version available

Fluorescent silver nanocluster DNA probes for multiplexed detection using microfluidic capillary electrophoresis

J. T. Del Bonis-O'Donnell, D. K. Fygenson and S. Pennathur, Analyst, 2015, 140, 1609 DOI: 10.1039/C4AN01735H

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