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Two azide-functionalized tetraphenylethene derivatives with AIE features are synthesized and used as fluorescent agents for detecting S-phase DNA synthesis and cell proliferation based on EdU assay. Compared to the Alexa-azide dye, a commercial DNA bioprobe, the AIE fluorogens show better photostability and sensitivity, making them promising alternatives.

Graphical abstract: Photostable AIE fluorogens for accurate and sensitive detection of S-phase DNA synthesis and cell proliferation

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