Issue 5, 2022

Quantitative elemental bioimaging: an antibody-based double-labelling method to quantify the cell-specific distribution of silver nanoparticles in lung tissue sections

Abstract

Silver nanoparticles (Ag-NP) are contained in many consumer products although their uptake especially by respiration bears potential risks for human health. Quantitative data about the systemic distribution of Ag-NP at the cellular level is strongly required to better understand the mode of action and to create meaningful in vitro models. In this paper we present a novel approach to identify cells in tissue sections by mass spectrometry imaging. Starting with a specific antibody bound to a cell marker protein we employed a double labelling strategy: a conventional secondary fluorescent antibody staining is followed by a labelling with a colloidal gold-coupled antibody directed against the same primary antibody. This allows identification of, e.g., regions of interest with conventional fluorescence microscopy and, thereafter, to detect identified cells by elemental mass spectrometry. The strategy circumvents the necessity to allocate mass-based signals to microscopic images. Unlike cost-intensive lanthanide-labelling the method is applicable to monoclonal and polyclonal primary antibodies. With this approach we confirmed the uptake of silver nanoparticles in alveolar macrophages and alveolar septal structures.

Graphical abstract: Quantitative elemental bioimaging: an antibody-based double-labelling method to quantify the cell-specific distribution of silver nanoparticles in lung tissue sections

Article information

Article type
Paper
Submitted
28 दिसम्बर 2021
Accepted
29 मार्च 2022
First published
30 मार्च 2022

J. Anal. At. Spectrom., 2022,37, 1084-1089

Quantitative elemental bioimaging: an antibody-based double-labelling method to quantify the cell-specific distribution of silver nanoparticles in lung tissue sections

O. Reifschneider, F. Blaske, A. Vennemann, C. Köppen, M. Sperling, U. Karst and M. Wiemann, J. Anal. At. Spectrom., 2022, 37, 1084 DOI: 10.1039/D1JA00466B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements