Themed collection Metallomics Recent HOT articles

Molecular mechanisms in the body regulate the cytotoxic properties of titanium(IV) such that Ti(IV) could be bioavailable for cellular functions.

Fungal pathogens of humans acquire iron by high affinity reductive uptake, the use of siderophores, and by heme acquisition. These systems contribute to virulence to different extents in the pathogens.

Copper (Cu) is indispensible for growth and development of human organisms.

The lysosomal system operates as a focal point where a number of important physiological processes such as endocytosis, autophagy and nutrient sensing converge.

Metal ions play crucial roles in numerous biological processes, facilitating biochemical reactions by binding to various proteins.

Mass spectrometry imaging is being increasingly used in metal-based anticancer drug development to study elemental and/or molecular drug distributions in different biological systems.

The metal transportome of Cupriavidus metallidurans. The cell of the Gram-negative bacterium is shown with the outer membrane (outermost grey line), the inner membrane lining the cytoplasm (light yellow) and the periplasm in-between. The number of metal atoms per cell is shown in the middle of the cell. Metal uptake and efflux systems for transition metal are indicated.

The insertion of copper into bacterial cuproenzymes in vivo does not always require a copper-binding metallochaperone – why?

Facultative trace element hyperaccumulation in animals is reviewed, examining mechanisms of uptake and accumulation, and biological roles.

Metal ions and complexes can interfere with the transcellular water flow but also with the cellular transport of glycerol and hydrogen peroxide, via inhibition of the ubiquitous aquaporin channels.

Nitration of human MnSOD at active site Tyr34 represents a biologically-relevant oxidative post-translational modification that causes enzyme inactivation.

Data on the location of alkaline and alkaline earth ions at RNA from crystallography, spectroscopy and computational modeling are reviewed.

Copper nitrite reductases (CuNiRs) catalyse the reduction of nitrite to nitric oxide as part of the denitrification pathway. In this review, we describe insights into CuNiR function from structural studies.

The biosyntheses of a family of enterobactin variants – amino acid substitution, insertion, and derivatization, and macrolactone expansion – is reviewed.

Iron efflux transporters play a critical role in protecting cells from iron intoxication.

Intestinal inflammation dysregulates iron metabolism and impairs erythropoiesis, leading to the development of anemia.

Bacteria produce the primordial antibiotic MAs(III) with the arsM gene, and other bacteria have arsI, arsH or arsP resistance genes.

We know that blood plasma contains many proteins and also other components that bind copper.

This critical review focuses on one possible link between the cellular biology of lead and its neurotoxic effects: the link between Pb²⁺ substitution for Zn²⁺ in zinc-finger proteins and mental illness in adulthood.

Platelets are known to be activated by exogenous zinc. Herein we discuss the potential routes for zinc entry and the role of zinc–protein interactions in platelet activation.

A review of iron and cadmium transport by renal tubules highlighting common pathways and differences with their implications for health and disease.

MhuD forms an enzymatically-active 1 : 1 complex with heme at nanomolar concentrations of labile heme and an inactive 1 : 2 complex at micromolar concentrations.

Coordination environment and conformational consequences of Cu(I) binding at Met-X₃-Met site of AcαS.

A single nucleotide polymorphism (rs10455) in the last exon of the Dcytb gene in C282Y hemochromatosis subjects exhibited increased ferric reductase activity in transgenic CHO cells.

This study provides new insights into understanding the interplay between ER quality control and copper transport mechanisms in Wilson disease.

A unified approach to chemical imaging was used to spatially profile essential bio-elements in Caenorhabditis elegans.

Mercury uptake by E. coli in the presence of excess cysteine is facilitated by the biodegradation of cysteine to sulfide and the formation of mercury sulfide species. The deletion of a key cysteine desulfhydrase gene (decR) limits the uptake of mercury in the presence of excess cysteine.

Bacteriophage T5 endolysin could be activated by Ca²⁺ in the periplasm of the host cell, thereby promoting bacterial lysis.

Iron uptake into mammalian ferritins reveals oxygen (but not iron) saturation kinetics and physiologically relevant K_m,O2 values.

Cu(II)–TETA is transported across the cell membrane via an unknown ATP-dependent and CTR1-independent process.

Ruthenium arene anticancer complexes bind to human peroxiredoxin I, leading to oxidation of thiolate and failure of forming a disulfide bond.

The histone H1 readily reacts with cisplatin and the adduct can form H1–cisplatin–DNA ternary complexes. Interestingly, cisplatin is more prone to form H1–Pt–DNA ternary complexes than trans-oriented platinum agents.

A stable gold(I) biscarbene complex with improved activity as a TrxR inhibitor and cytotoxic metallodrug.

In C. metallidurans, a network of 11 extracytoplasmic function sigma factors forms the third pillar of metal homeostasis acting in addition to the metal transportome and metal repositories as the first and second pillar.

Several Pt(IV) prodrugs conjugated with a glutathione S-transferase (GST) inhibitor were studied with the purpose of preventing the metastasis of osteosarcoma.

Mice treated daily with MT2 (but not MT1) showed a significant amelioration of the clinical course of experimental autoimmune encephalomyelitis (EAE).

Copper deficiency reprograms mitochondrial metabolism, which becomes less oxidative, to favor cell expansion over cell differentiation.

The binding of Cu, Fe or Zn to alpha-synuclein has been implicated in neurodegenerative disease, such as Parkinson's.

The target spectrum and cellular effects of a metallo-prodrug can be separated from its activated species by time-dependent shotgun proteomics.

We cataloged the zinc proteome of Saccharomyces cerevisiae and analyzed how it responds to changing zinc status.

The metal (red) bound form of calprotectin (green) binds Borrelia burgdorferi and inhibits pathogen growth without metal withholding.

Alzheimer's disease (AD) involves a number of factors including an anomalous interaction of copper with the amyloid peptide (Aβ), inducing oxidative stress with radical oxygen species (ROS) production through a three-step cycle in which O₂ is gradually reduced to superoxide, oxygen peroxide and finally OH radicals.

Overexpression of HIF2α via miR-182-5p suppression is involved in arsenic-induced malignant transformation of human renal epithelial cells.

Copper accumulation in the Atp7b^−/− model of Wilson disease impacts zinc distribution.

Rapeseed MT4 only folds properly in the presence of Zn²⁺ and thus may serve as a selectivity filter for metal accumulation in plant embryos.

The NMR solution structure of a Pseudomonas metallothionein reveals a different binding capacity for Zn^II and Cd^II ions that results in two novel metal-cluster topologies. Replacement of a non-coordinating residue by histidine decreases the kinetic lability of the cluster. All three structures reported show an identical protein fold.

In this work we compiled a comprehensive list of all human iron-proteins based on bioinformatics predictions and analyzed their functional roles, distribution within cell compartments and involvement in disease.

Pb²⁺ binds C2 domains with high affinity, desensitizes them to Ca²⁺, and supports their membrane interactions.

Mitochondrial Mg channel MRS2 overexpression influences Mg concentration and sensitivity to apoptosis.

The labile iron pool in blood consists of complexes with masses of 400–2500 Daltons that are not ferric citrate.

Coregistering quantitative high-resolution metal imaging with fluorescence imaging identifies copper accumulation in megamitochondria within intact neuroretinal slices.

Nickel(II) inhibits Tet-mediated oxidation of DNA 5-methylcytosine in mammalian cells.

Metal-based drugs are gaining momentum as a rapidly developing area of medicinal inorganic chemistry.

A cytosolic copper storage protein has been identified in Streptomyces lividans and plays a role in copper tolerance once the first layer of copper resistance becomes saturated.

Herein, we measure the Ca isotope ratios (⁴⁴Ca/⁴²Ca and ⁴³Ca/⁴²Ca) in serum and bone samples collected from rats with chronic kidney disease (CKD) or diabetes mellitus (DM).

Investigation of the mechanisms of mitochondrial metal binding to frataxin in vitro.

BTS family members negatively regulate the Fe deficiency response; mutants have increased Fe levels and tolerance to Fe deficiency.

A highly-simplified scenario of LF/U-fOPN interaction. The U content of the U-fOPN complexes refers to the CE-ICP/MS experiments.

Vanadium compounds were studied in recent years by considering them as a representative of a new class of non-platinum metal anticancer drugs.

One dual-function (2) and one first-generation (9) conjugate of the Fe(III) chelator desferrioxamine B (DFOB, 1) showed significant rescue of neurons in the MPTP mouse model of Parkinson's disease.

The BfrB:Bfd interaction enables a dynamic equilibrium between free cytosolic Fe²⁺ and Fe³⁺ in BfrB, which functions as a buffer to oppose large fluctuations of free cytosolic Fe²⁺.

Evaluation of the effect of metal exposure (cadmium/copper) on metabolic pathways of rice.

Speciation resulting from 20 bimolecular, sequential reactions involving 20 Cu(I) ions binding to metallothionein at different pH-values is reported.

The siderophore pre-acinetobactin undergoes a facile 5-exo-tet cyclization to enhance iron acquisition and virulence of the pathogenic producer Acinetobacter baumannii.

Unique long-range DNA interstrand cross-links of a dinuclear platinum complex formed in the 3′–3′ direction affect its antitumor effects.

SlyD activates the release of nickel and blocks the release of zinc from the high-affinity metal site of HypB.

Synchrotron X-ray fluorescence microscopy (SXRF) data reveal that metalloprotein inhibitors (MPi) do not significantly perturb cellular Zn content or distribution.

Knowledge about mammalian selenoproteins is increasing.

Aβ oxidation due to HO˙ production by Cu–Aβ/ascorbate changes copper coordination and leads to the self-enhancement of HO˙ production and release.

Copper loss in ischemic myocardium makes a critical contribution to the suppression of HIF-1-regulated angiogenesis.