Introduction to advanced separation
Zhen Liu, Wenwan Zhong and Takuya Kubo introduce the Analytical Methods themed collection on advanced separation.
Metal–organic frameworks as advanced materials for sample preparation of bioactive peptides
This minireview summarizes the recent advances in MOF-based affinity materials for the sample preparation of bioactive peptides.
Advances in epitope molecularly imprinted polymers for protein detection: a review
Epitope molecularly imprinted polymers (EMIPs) are novel imprinted materials using short characteristic peptides as templates rather than entire proteins.
Recent technical progress in sample preparation and liquid-phase separation-mass spectrometry for proteomic analysis of mass-limited samples
Recent development of sample preparation methods with nanoliter processing volumes, ultrasensitive LC-MS/MS and CZE-MS/MS systems for proteomics of mass-limited samples.
Methods for isolation of messenger RNA from biological samples
mRNA molecules are critical components in living organisms. Isolation of highly pure mRNA is the first step in many biological researches. This review focuses on the methodologies and protocols for isolating mRNA populations.
Chiral porous organic frameworks and their application in enantioseparation
The application of CMOFs and CCOFs as CSPs for chromatographic chiral separation.
Fluorescent detection of target proteins via a molecularly imprinted hydrogel
A molecularly imprinted hydrogel with a PEG-fluorescent monomer was used for the selective detection of a target protein.
Simultaneous metabolomics and proteomics analysis of plasma-derived extracellular vesicles
A tandem extraction strategy was established to obtain metabolites and proteins from the same batch of extracellular vesicles (EVs) simultaneously, enabling the multi-omics differential analysis of exosomes and microvesicles from human plasma.
A new urinary exosome enrichment method by a combination of ultrafiltration and TiO2 nanoparticles
The workflow of separation and enrichment of exosomes by ultrafiltration–TiO2. We proposed a new strategy for facile exosome isolation from human urine by utilizing the ultrafiltration technique and TiO2, which can significantly reduce urine volumes, increase exposure of the material to exosomes in urine and obtain high purity exosomes.
Development of the C12Im-Cl-assisted method for rapid sample preparation in proteomic application
C12Im-Cl could work as a protein solubilizer compatible with trypsin digestion and reduced the protein digestion time to one hour.
Quantitative proteomics of epigenetic histone modifications in MCF-7 cells under estradiol stimulation
SILAC-based quantitative proteomics strategy deciphers the mechanism of epigenetic regulation for breast cancer cell under estradiol stimulation.
Highly selective enrichment and direct determination of imazethapyr residues from milk using magnetic solid-phase extraction based on restricted-access molecularly imprinted polymers
RAM-MIPs was prepared as MSPE material for selective extraction of trace IMs and possessed satisfactory protein exclusion properties.
ZnO/SiO2 core/shell nanowires for capturing CpG rich single-stranded DNAs
Modification of the surface of ZnO nanowires through atomic layer deposition (ALD) for the fabrication of a ZnO/SiO2 (core/shell) nanowire microfluidic device for capturing CpG-rich single-stranded DNAs (ssDNAs).
Probing the methotrexate–protein interactions by proteomics and thermostability assay for drug resistance study
Quantitative proteomics combined with thermostability assay provide a novel strategy for the study of mechanisms on drug action and resistance.
About this collection
A collection of papers and reviews on the topics of advanced materials and substances for separation, microseparation, multidimensional separation, 3D printing for separation, electrophoretic cytometry, and advanced separation techniques for challenging applications (such as food safety and -omics studies).
This collection is Guest Edited by Analytical Methods Associate Editor, Zhen Liu (Nanjing University, China), Wenwan Zhong (University of California, Riverside, USA) and Takuya Kubo (Kyoto University, Japan).