Issue 22, 2023

Hydrophilic magnetic host–guest Ti–phenolic networks: a promising material for the highly sensitive enrichment of glycopeptides and phosphopeptides

Abstract

Protein glycosylation and phosphorylation are the two most important post-translational modifications, which play a vital role in physiological and pathological processes. Before the comprehensive characterization of glycoproteome/phosphoproteome through the mass spectrometry (MS) technique, it is necessary to perform a highly specific enrichment procedure because glycoproteins/phosphoproteins inherently occur in low abundances. Herein, we have reported a novel magnetic β-cyclodextrin-based host–guest Ti–phenolic network material, focusing on the simultaneous enrichment of glycopeptides/phosphopeptides via hydrophilic interaction chromatography and immobilized metal ion chromatography. Ti ions and glutathione-derived adamantine were introduced by metal–phenolic interactions and host–guest interactions. The material possesses biocompatibility, good hydrophilicity, strong magnetic response, metal chelation effect, and demonstrates an excellent enrichment ability towards glycopeptides/phosphopeptides. Combined with MS detection, high sensitivity (0.035/0.01 fmol for IgG/β-casein) and good reusability (6 times) were achieved. In addition, its outstanding specificity was validated in quantities as low as 500 : 1 : 1 for BSA : IgG : β-casein (m/m/m). Benefiting from these merits, the adsorbent material was successfully used for the simultaneous enrichment of phosphopeptides/glycopeptides from human serum and HeLa cell lysate and can be expected to exhibit great applicability for precious and small amounts of biosamples in the study of glycoproteomics/phosphoproteomics.

Graphical abstract: Hydrophilic magnetic host–guest Ti–phenolic networks: a promising material for the highly sensitive enrichment of glycopeptides and phosphopeptides

Supplementary files

Article information

Article type
Paper
Submitted
11 août 2022
Accepted
09 mai 2023
First published
24 mai 2023

J. Mater. Chem. B, 2023,11, 4874-4881

Hydrophilic magnetic host–guest Ti–phenolic networks: a promising material for the highly sensitive enrichment of glycopeptides and phosphopeptides

Y. Wang, P. Li, W. Xu, D. Zhang and Q. Jia, J. Mater. Chem. B, 2023, 11, 4874 DOI: 10.1039/D2TB01708C

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