Issue 31, 2022

Design and applications of water irradiation devoid RF pulses for ultra-high field biomolecular NMR spectroscopy

Abstract

Water suppression is of paramount importance for many biological and analytical NMR spectroscopy applications. Here, we report the design of a new set of binomial-like radio frequency (RF) pulses that elude water irradiation while exciting or refocusing the remainder of the 1H spectrum. These pulses were generated using a combination of an evolutionary algorithm and artificial intelligence. They display higher sensitivity relative to classical water suppression schemes and tunable water selectivity to avoid suppressing 1H resonances near the water signal. The broad bandwidth excitation obtained with these RF pulses makes them suitable for several NMR applications at high and ultra-high-field magnetic fields.

Graphical abstract: Design and applications of water irradiation devoid RF pulses for ultra-high field biomolecular NMR spectroscopy

Supplementary files

Article information

Article type
Communication
Submitted
14 avr. 2022
Accepted
19 juil. 2022
First published
19 juil. 2022

Phys. Chem. Chem. Phys., 2022,24, 18477-18481

Design and applications of water irradiation devoid RF pulses for ultra-high field biomolecular NMR spectroscopy

V. S. Manu, C. Olivieri, K. Pavuluri and G. Veglia, Phys. Chem. Chem. Phys., 2022, 24, 18477 DOI: 10.1039/D2CP01744J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements