Issue 35, 2017

Membrane interactions and antimicrobial effects of layered double hydroxide nanoparticles

Abstract

Membrane interactions are critical for the successful use of inorganic nanoparticles as antimicrobial agents and as carriers of, or co-actives with, antimicrobial peptides (AMPs). In order to contribute to an increased understanding of these, we here investigate effects of particle size (42–208 nm) on layered double hydroxide (LDH) interactions with both bacteria-mimicking and mammalian-mimicking lipid membranes. LDH binding to bacteria-mimicking membranes, extraction of anionic lipids, as well as resulting membrane destabilization, was found to increase with decreasing particle size, also translating into size-dependent synergistic effects with the antimicrobial peptide LL-37. Due to strong interactions with anionic lipopolysaccharide and peptidoglycan layers, direct membrane disruption of both Gram-negative and Gram-positive bacteria is suppressed. However, LDH nanoparticles cause size-dependent charge reversal and resulting flocculation of both liposomes and bacteria, which may provide a mechanism for bacterial confinement or clearance. Taken together, these findings demonstrate a set of previously unknown behaviors, including synergistic membrane destabilization and dual confinement/killing of bacteria through combined LDH/AMP exposure, of potential therapeutic interest.

Graphical abstract: Membrane interactions and antimicrobial effects of layered double hydroxide nanoparticles

Supplementary files

Article information

Article type
Paper
Submitted
25 avr. 2017
Accepted
28 juin 2017
First published
06 juil. 2017

Phys. Chem. Chem. Phys., 2017,19, 23832-23842

Membrane interactions and antimicrobial effects of layered double hydroxide nanoparticles

S. Malekkhaiat Häffner, L. Nyström, R. Nordström, Z. P. Xu, M. Davoudi, A. Schmidtchen and M. Malmsten, Phys. Chem. Chem. Phys., 2017, 19, 23832 DOI: 10.1039/C7CP02701J

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