Issue 12, 2007
From the journal:

Chemical Communications

Quantitative detection of protein expression in single cells using droplet microfluidics†

A. Huebner,ab   M. Srisa-Art,c   D. Holt,a   C. Abell,a   F. Hollfelder,b   A. J. deMelloc  and  J. B. Edelcd  

a Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK

b Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge, UK
E-mail: fh111@cam.ac.uk

c Department of Chemistry, Imperial College London, South Kensington, UK
E-mail: a.demello@imperial.ac.uk

d Institute of Biomedical Engineering, Imperial College London, South Kensington, UK
E-mail: joshua.edel@imperial.ac.uk

Abstract

We demonstrate that single cells can be controllably compartmentalized within aqueous microdroplets; using such an approach we perform high-throughput screening by detecting the expression of a fluorescent protein in individual cells with simultaneous measurement of droplet size and cell occupancy.

Graphical abstract: Quantitative detection of protein expression in single cells using droplet microfluidics

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Article information

DOI
https://doi.org/10.1039/B618570C
Article type
Communication
Submitted
19 déc. 2006
Accepted
16 janv. 2007
First published
26 janv. 2007

Chem. Commun., 2007, 1218-1220

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Quantitative detection of protein expression in single cells using droplet microfluidics

A. Huebner, M. Srisa-Art, D. Holt, C. Abell, F. Hollfelder, A. J. deMello and J. B. Edel, Chem. Commun., 2007, 1218 DOI: 10.1039/B618570C

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