Issue 19, 2024

Reinvestigation of the internal glycan rearrangement of Lewis a and blood group type H1 epitopes

Abstract

Protonated ions of fucose-containing oligosaccharides are prone to undergo internal glycan rearrangement which results in chimeric fragments that obfuscate mass-spectrometric analysis. Lack of accessible tools that would facilitate systematic analysis of glycans in the gas phase limits our understanding of this phenomenon. In this work, we use density functional theory modeling to interpret cryogenic IR spectra of Lewis a and blood group type H1 trisaccharides and to establish whether these trisaccharides undergo the rearrangement during gas-phase analysis. Structurally unconstrained search reveals that none of the parent ions constitute a thermodynamic global minimum. In contrast, predicted collision cross sections and anharmonic IR spectra provide a good match to available experimental data which allowed us to conclude that fucose migration does not occur in these antigens. By comparing the predicted structures with those obtained for Lewis x and blood group type H2 epitopes, we demonstrate that the availability of the mobile proton and a large difference in the relative stability of the parent ions and rearrangement products constitute the prerequisites for the rearrangement reaction.

Graphical abstract: Reinvestigation of the internal glycan rearrangement of Lewis a and blood group type H1 epitopes

Supplementary files

Article information

Article type
Paper
Submitted
15 sept. 2023
Accepted
17 avr. 2024
First published
24 avr. 2024

Phys. Chem. Chem. Phys., 2024,26, 14160-14170

Reinvestigation of the internal glycan rearrangement of Lewis a and blood group type H1 epitopes

V. Kontodimas, M. Yaman, K. Greis, M. Lettow, K. Pagel and M. Marianski, Phys. Chem. Chem. Phys., 2024, 26, 14160 DOI: 10.1039/D3CP04491B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements