Strong fluorescence-detected two-photon circular dichroism of chiral gold nanoclusters

Abstract

Progress in syntheses and understanding of the intriguing properties of chiral noble metal nanoclusters sparks interest to extend investigations of their chiroptical response to the nonlinear optics regime. We present a quantitative determination of two-photon circular dichroism of chiral gold nanoclusters with ATT and L- or D-Arg ligands (ATT = 6-aza-2-thiotymine and Arg = arginine). Introduction of arginine ligands enables the formation of two enantiomers of the nanoclusters, with strong chiroptical effects in both linear and nonlinear regime. We present two-photon absorption and luminescent properties measured in a wide range of wavelengths, with the two-photon absorption cross section reaching 1743 GM and two-photon brightness ∼1102 GM at 825 nm. We report strong, 245-fold enhancement of the two-photon circular dichroism of nanoclusters with respect to the one-photon absorption counterpart – the dissymmetry factor. The presence of multiple advantages of nanoclusters: high fluorescence quantum yield, strong nonlinear optical properties and well-controlled chirality is a powerful combination for applications of such clusters in multiphoton microscopy.