Issue 39, 2023

Microdialysis on-chip crystallization of soluble and membrane proteins with the MicroCrys platform and in situ X-ray diffraction case studies

Abstract

This work describes the design and operation of a prototype platform (MicroCrys) developed to automate the on-chip dialysis crystallization of proteins via chemical composition and temperature control, facilitating the optimization of crystallization conditions and the exploration of protein phase diagrams. A custom-built computer software (LabVIEW) displaying a user-friendly graphical interface has been designed to manipulate all the separate functionalities of the MicroCrys platform. These include visualization and recording, fluidic components for mixing, circulating and dynamically exchanging the crystallization solutions in the dialysis chip and a customized metallic support for thermal regulation via the Peltier effect. Two model soluble proteins, hen egg white lysozyme (HEWL) and thaumatin from Thaumatococcus daniellii, were used to exemplify the competence of MicroCrys for growing high-quality protein crystals for in situ X-ray diffraction experiments at room temperature. HEWL was also used to validate the compatibility of the dialysis chips with the lipid cubic phase (LCP) crystallization method and the microcrystals were used for room temperature in situ synchrotron serial X-ray crystallography (SSX). Finally, SERCA (sarco/endoplasmic reticulum Ca2+-ATPase) from Oryctolagus cuniculus has been used as a case study to demonstrate the feasibility of on-chip microdialysis crystallization of membrane proteins.

Graphical abstract: Microdialysis on-chip crystallization of soluble and membrane proteins with the MicroCrys platform and in situ X-ray diffraction case studies

Supplementary files

Article information

Article type
Paper
Submitted
04 mai 2023
Accepted
02 sept. 2023
First published
15 sept. 2023

CrystEngComm, 2023,25, 5513-5523

Microdialysis on-chip crystallization of soluble and membrane proteins with the MicroCrys platform and in situ X-ray diffraction case studies

S. Jaho, Y. Sallaz-Damaz and M. Budayova-Spano, CrystEngComm, 2023, 25, 5513 DOI: 10.1039/D3CE00466J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements