Issue 35, 2023

Electrochemical detection of glycoproteins using boronic acid-modified metal–organic frameworks as dual-functional signal reporters

Abstract

The sensitive analysis of glycoproteins is of great importance for early diagnosis and prognosis of diseases. In this work, a sandwich-type electrochemical aptasensor was developed for the detection of glycoproteins using 4-formylphenylboric acid (FPBA)-modified Cu-based metal–organic frameworks (FPBA-Cu-MOFs) as dual-functional signal probes. The target captured by the aptamer-modified electrode allowed the attachment of FPBA-Cu-MOFs based on the interaction between boronic acid and glycan on glycoproteins. Large numbers of Cu2+ ions in FPBA-Cu-MOFs produced an amplified signal for the direct voltammetric detection of glycoproteins. The electrochemical aptasensor showed a detection limit as low as 6.5 pg mL−1 for prostate specific antigen detection. The method obviates the use of antibody and enzymes for molecular recognition and signal output. The dual-functional MOFs can be extended to the design of other biosensors for the determination of diol-containing biomolecules in clinical diagnosis.

Graphical abstract: Electrochemical detection of glycoproteins using boronic acid-modified metal–organic frameworks as dual-functional signal reporters

Article information

Article type
Paper
Submitted
09 juil. 2023
Accepted
09 août 2023
First published
11 août 2023

Anal. Methods, 2023,15, 4452-4458

Electrochemical detection of glycoproteins using boronic acid-modified metal–organic frameworks as dual-functional signal reporters

Y. Chang, Y. Chen, M. Wu, L. Liu and Q. Song, Anal. Methods, 2023, 15, 4452 DOI: 10.1039/D3AY01164J

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