Issue 37, 2022

Protein precoating modulates biomolecular coronas and nanocapsule–immune cell interactions in human blood

Abstract

The biomolecular corona that forms on particles upon contact with blood plays a key role in the fate and utility of nanomedicines. Recent studies have shown that precoating nanoparticles with serum proteins can improve the biocompatibility and stealth properties of nanoparticles. However, it is not fully clear how precoating influences biomolecular corona formation and downstream biological responses. Herein, we systematically examine three precoating strategies by coating bovine serum albumin (single protein), fetal bovine serum (FBS, mixed proteins without immunoglobulins), or bovine serum (mixed proteins) on three nanoparticle systems, namely supramolecular template nanoparticles, metal–phenolic network (MPN)-coated template (core–shell) nanoparticles, and MPN nanocapsules (obtained after template removal). The effect of protein precoating on biomolecular corona compositions and particle–immune cell interactions in human blood was characterized. In the absence of a pre-coating, the MPN nanocapsules displayed lower leukocyte association, which correlated to the lower amount (by 2–3 fold) of adsorbed proteins and substantially fewer immunoglobulins (more than 100 times) in the biomolecular corona relative to the template and core–shell nanoparticles. Among the three coating strategies, FBS precoating demonstrated the most significant reduction in leukocyte association (up to 97% of all three nanoparticles). A correlation analysis highlights that immunoglobulins and apolipoproteins may regulate leukocyte recognition. This study demonstrates the impact of different precoating strategies on nanoparticle–immune cell association and the role of immunoglobulins in bio–nano interactions.

Graphical abstract: Protein precoating modulates biomolecular coronas and nanocapsule–immune cell interactions in human blood

Supplementary files

Article information

Article type
Paper
Submitted
28 mars 2022
Accepted
09 juin 2022
First published
09 juin 2022

J. Mater. Chem. B, 2022,10, 7607-7621

Protein precoating modulates biomolecular coronas and nanocapsule–immune cell interactions in human blood

S. Li, Y. Ju, J. Zhou, M. Faria, C. Ang, A. J. Mitchell, Q. Zhong, T. Zheng, S. J. Kent and F. Caruso, J. Mater. Chem. B, 2022, 10, 7607 DOI: 10.1039/D2TB00672C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements