Non-competitive fluorescence polarization immunosensing for CD9 detection using a peptide as a tracer

Kazuki Takahashi; Shunsuke Chida; Thanawat Suwatthanarak; Mikiko Iida; Min Zhang; Mao Fukuyama; Masatoshi Maeki; Akihiko Ishida; Hirofumi Tani; Takao Yasui; Yoshinobu Baba; Akihide Hibara; Mina Okochi; Manabu Tokeshi

doi:10.1039/D2LC00224H

Non-competitive fluorescence polarization immunosensing for CD9 detection using a peptide as a tracer

Kazuki Takahashi,^a Shunsuke Chida,^a Thanawat Suwatthanarak,^b Mikiko Iida,^c Min Zhang,^c Mao Fukuyama,

^d Masatoshi Maeki,

^ef Akihiko Ishida,

^e Hirofumi Tani,

^e Takao Yasui,

^cfg Yoshinobu Baba,^cgh Akihide Hibara,

^d Mina Okochi

^b and Manabu Tokeshi

*^eghi

Author affiliations

* Corresponding authors

^a Graduate School of Chemical Sciences and Engineering, Hokkaido University, Kita 13 Nishi 8, Kita-ku, Sapporo 060-8628, Japan

^b Department of Chemical Science & Engineering, Tokyo Institute of Technology, 2-12-2 O-okayama, Meguro-ku, Tokyo 152-8552, Japan

^c Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan

^d Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan

^e Division of Applied Chemistry, Faculty of Engineering, Hokkaido University, Kita 13 Nishi 8, Kita-ku, Sapporo 060-8628, Japan
E-mail: tokeshi@eng.hokudai.ac.jp
Fax: +81 11 706 6745
Tel: +81 11 706 6744

^f Japan Science and Technology Agency (JST), PRESTO, 4-1-8, Honcho, Kawaguchi, Saitama 332-0012, Japan

^g Institute of Nano-Life Systems, Institute of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan

^h Institute for Quantum Life Science, National Institutes for Quantum Science and Technology (QST), 4-9-1, Anagawa, Inage-ku, Chiba 263-0024, Japan

ⁱ Innovative Research Center for Preventive Medical Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan

Abstract

This paper is the first report of a non-competitive fluorescence polarization immunoassay (NC-FPIA) using a peptide as a tracer. The NC-FPIA can easily and quickly quantify the target after simply mixing them together. This feature is desirable for point-of-need applications such as clinical diagnostics, infectious disease screening, on-site analysis for food safety, etc. In this study, the NC-FPIA was applied to detect CD9, which is one of the exosome markers. We succeeded in detecting not only CD9 but also CD9 expressing exosomes derived from HeLa cells. This method can be applied to various targets if a tracer for the target can be prepared, and expectations are high for its future uses.

This article is part of the themed collection: Miniaturised Sensors & Diagnostics

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Non-competitive fluorescence polarization immunosensing for CD9 detection using a peptide as a tracer

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Non-competitive fluorescence polarization immunosensing for CD9 detection using a peptide as a tracer

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