Issue 3, 2022
From the journal:

RSC Chemical Biology

Dependence of click-SELEX performance on the nature and average number of modified nucleotides†

Julia Siegl,a   Olga Plückthuna  and  Günter Mayer ORCID logo *ab  

* Corresponding authors

a Chemical Biology & Chemical Genetics, Life and Medical Sciences (LIMES) Institute, University of Bonn, Gerhard-Domagk-Str. 1, 53121 Bonn, Germany
E-mail: gmayer@uni-bonn.de

b Center of Aptamer Research & Development (CARD), University of Bonn, Gerhard-Domagk-Str. 1, 53121 Bonn, Germany

Abstract

The click-SELEX procedure enables the identification of nucleobase-modified aptamers in which chemical entities are introduced by a copper(I)-catalysed alkyne-azide ‘click’ reaction. Here we report on the impact of modified nucleobases on PCR conditions and the average amount of modified nucleobases on click-SELEX performance. We demonstrate click-SELEX being strongly dependent on which and on how many modifications are used. However, when using C3-GFP the number of modifications did not impact the overall success of the selection procedure.

Graphical abstract: Dependence of click-SELEX performance on the nature and average number of modified nucleotides

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Article information

DOI
https://doi.org/10.1039/D2CB00012A
Article type
Communication
Submitted
14 janv. 2022
Accepted
17 févr. 2022
First published
21 févr. 2022
This article is Open Access
Creative Commons BY-NC license

RSC Chem. Biol., 2022,3, 288-294

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Dependence of click-SELEX performance on the nature and average number of modified nucleotides

J. Siegl, O. Plückthun and G. Mayer, RSC Chem. Biol., 2022, 3, 288 DOI: 10.1039/D2CB00012A

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