Issue 15, 2022

Recent advances in biological detection with rolling circle amplification: design strategy, biosensing mechanism, and practical applications

Abstract

Rolling circle amplification (RCA) is a simple and isothermal DNA amplification technique that is used to generate thousands of repeating DNA sequences using circular templates under the catalysis of DNA polymerase. Compared to alternating temperature nucleic acid amplification such as polymerase chain reaction (PCR) amplification, RCA is more suitable for on-spot detection without the need for an expensive thermal cycler. In this study, the principle and classification of RCA are introduced, and the applications of RCA in the detection of pathogenic bacteria, nucleic acid tumor markers, viruses, and proteins are reviewed. Finally, the perspectives of RCA in biological detection are anticipated. The RCA method has a great potential for biological detection. This review aims to provide references for the further development and application of the RCA technique in biosensors.

Graphical abstract: Recent advances in biological detection with rolling circle amplification: design strategy, biosensing mechanism, and practical applications

Article information

Article type
Minireview
Submitted
30 mars 2022
Accepted
13 juin 2022
First published
13 juin 2022

Analyst, 2022,147, 3396-3414

Recent advances in biological detection with rolling circle amplification: design strategy, biosensing mechanism, and practical applications

Y. Gao, K. Huang, F. Wang, Y. Hou, J. Xu and G. Li, Analyst, 2022, 147, 3396 DOI: 10.1039/D2AN00556E

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