Issue 86, 2021

Turn on chemiluminescence-based probes for monitoring tyrosinase activity in conjunction with biological thiols

Abstract

We report a chemiluminescent probe (CLPT1) that permits the paired detection of tyrosinase (Tyr) and biological thiols. Tyr only leads to a poor chemiluminescence response, a finding ascribed to the formation of a stable o-benzoquinone intermediate. The addition of glutathione (GSH), or ascorbate to the o-benzoquinone intermediate results in thiol conjugation or reduction to this intermediate, respectively. This produces a strong chemiluminescence response. Thiol co-dependence was demonstrated in live cells using the cell permeable analogue, CLPT3. The present chemiluminescence-based strategy allows the concurrent detection of tyrosinase activity and biological thiols.

Graphical abstract: Turn on chemiluminescence-based probes for monitoring tyrosinase activity in conjunction with biological thiols

Supplementary files

Article information

Article type
Communication
Submitted
15 sept. 2021
Accepted
04 oct. 2021
First published
04 oct. 2021

Chem. Commun., 2021,57, 11386-11389

Turn on chemiluminescence-based probes for monitoring tyrosinase activity in conjunction with biological thiols

O. Shelef, A. C. Sedgwick, S. Pozzi, O. Green, R. Satchi-Fainaro, D. Shabat and J. L. Sessler, Chem. Commun., 2021, 57, 11386 DOI: 10.1039/D1CC05217A

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