Issue 12, 2021

Separation of distinct exosome subpopulations: isolation and characterization approaches and their associated challenges

Abstract

Exosomes are nano-sized extracellular vesicles that serve as a communications system between cells and have shown tremendous promise as liquid biopsy biomarkers in diagnostic, prognostic, and even therapeutic use in different human diseases. Due to the natural heterogeneity of exosomes, there is a need to separate exosomes into distinct biophysical and/or biochemical subpopulations to enable full interrogation of exosome biology and function prior to the possibility of clinical translation. Currently, there exists a multitude of different exosome isolation and characterization approaches which can, in limited capacity, separate exosomes based on biophysical and/or biochemical characteristics. While notable reviews in recent years have reviewed these approaches for bulk exosome sorting, we herein present a comprehensive overview of various conventional technologies and modern microfluidic and nanotechnological advancements towards isolation and characterization of exosome subpopulations. The benefits and limitations of these different technologies to improve their use for distinct exosome subpopulations in clinical practices are also discussed. Furthermore, an overview of the most commonly encountered technical and biological challenges for effective separation of exosome subpopulations is presented.

Graphical abstract: Separation of distinct exosome subpopulations: isolation and characterization approaches and their associated challenges

Article information

Article type
Minireview
Submitted
06 janv. 2021
Accepted
09 mai 2021
First published
10 mai 2021

Analyst, 2021,146, 3731-3749

Separation of distinct exosome subpopulations: isolation and characterization approaches and their associated challenges

K. Singh, R. Nalabotala, K. M. Koo, S. Bose, R. Nayak and M. J. A. Shiddiky, Analyst, 2021, 146, 3731 DOI: 10.1039/D1AN00024A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements