Issue 4, 2021

Multicolour chemical imaging of plant tissues with hyperspectral stimulated Raman scattering microscopy

Abstract

Recent development of stimulated Raman scattering (SRS) microscopy allows for label-free biological imaging with chemical specificity based on molecular-vibrational signatures. In particular, hyperspectral SRS imaging can acquire a molecular-vibrational spectrum at each pixel, allowing us not only to investigate the spectral difference of various biological molecules but also to discriminate different constituents based on their spectral difference. However, the number of constituents discriminated in previous label-free SRS imaging was limited to four because of the subtleness of spectral difference. Here, we report hyperspectral SRS imaging of plant tissues including leaves of Camellia japonica, roots of Arabidopsis thaliana, and thalli of a liverwort Marchantia polymorpha L. We show that SRS can discriminate as many as six components in Marchantia polymorpha L. without labeling. Our results demonstrate the effectiveness of hyperspectral SRS imaging as a tool for label-free multicolour imaging analysis of various biomolecules in plant tissues.

Graphical abstract: Multicolour chemical imaging of plant tissues with hyperspectral stimulated Raman scattering microscopy

Article information

Article type
Paper
Submitted
04 nov. 2020
Accepted
11 déc. 2020
First published
14 déc. 2020

Analyst, 2021,146, 1234-1238

Author version available

Multicolour chemical imaging of plant tissues with hyperspectral stimulated Raman scattering microscopy

T. Iino, K. Hashimoto, T. Asai, K. Kuchitsu and Y. Ozeki, Analyst, 2021, 146, 1234 DOI: 10.1039/D0AN02181D

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