Issue 1, 2019
From the journal:

Organic & Biomolecular Chemistry

Heterologous production of asperipin-2a: proposal for sequential oxidative macrocyclization by a fungi-specific DUF3328 oxidase†

Ying Ye,a   Taro Ozaki, ORCID logo *a   Myco Umemura,bc   Chengwei Liu,a   Atsushi Minamia  and  Hideaki Oikawa ORCID logo *a  

* Corresponding authors

a Department of Chemistry, Faculty of Science, Hokkaido University, Sapporo 060-0810, Hokkaido, Japan
E-mail: ozaki@sci.hokudai.ac.jp, hoik@sci.hokudai.ac.jp

b Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8566, Ibaraki, Japan

c Computational Bio Big Data Open Innovation Laboratory (CBBD-OIL), AIST, Tsukuba 305-8566, Ibaraki, Japan

Abstract

Asperipin-2a is a ribosomally synthesized and post-translationally modified peptide isolated from Asperigillus flavus. Herein, we report the heterologous production of asperipin-2a and determination of its absolute structure. Notably, the characteristic bicyclic structure was likely constructed by a single oxidase containing the DUF3328 domain.

Graphical abstract: Heterologous production of asperipin-2a: proposal for sequential oxidative macrocyclization by a fungi-specific DUF3328 oxidase

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Article information

DOI
https://doi.org/10.1039/C8OB02824A
Article type
Communication
Submitted
13 nov. 2018
Accepted
28 nov. 2018
First published
28 nov. 2018

Org. Biomol. Chem., 2019,17, 39-43

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Heterologous production of asperipin-2a: proposal for sequential oxidative macrocyclization by a fungi-specific DUF3328 oxidase

Y. Ye, T. Ozaki, M. Umemura, C. Liu, A. Minami and H. Oikawa, Org. Biomol. Chem., 2019, 17, 39 DOI: 10.1039/C8OB02824A

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