Reaction-based indicator displacement assay (RIA) for the development of a triggered release system capable of biofilm inhibition

Bethany L. Patenall; George T. Williams‡; Lauren Gwynne; Liam J. Stephens; Emma V. Lampard; Hollie J. Hathaway; Naing T. Thet; Amber E. Young; Mark J. Sutton; Robert D. Short; Steven D. Bull; Tony D. James; Adam C. Sedgwick; A. Toby A. Jenkins

doi:10.1039/C9CC07759F

Reaction-based indicator displacement assay (RIA) for the development of a triggered release system capable of biofilm inhibition†

Bethany L. Patenall,

‡^a George T. Williams‡,

^a Lauren Gwynne,

^a Liam J. Stephens,

^a Emma V. Lampard,

^a Hollie J. Hathaway,

^b Naing T. Thet,^a Amber E. Young,

^c Mark J. Sutton,

^d Robert D. Short,

^b Steven D. Bull,

^a Tony D. James,

*^a Adam C. Sedgwick

*^e and A. Toby A. Jenkins

*^a

Author affiliations

* Corresponding authors

^a Department of Chemistry, University of Bath, Bath, UK
E-mail: t.d.james@bath.ac.uk, A.T.A.Jenkins@bath.ac.uk

^b Department of Chemistry, Lancaster University, UK

^c The Scar Free Foundation Centre for Children's Burns Research, The Bristol Royal Hospital for Children, Bristol, UK

^d Public Health England, Porton Down, Salisbury, Wiltshire SP4 0JG, UK

^e Department of Chemistry, University of Texas at Austin, 105 East 24th Street A5300, Austin, Texas 78712-1224, USA
E-mail: a.c.sedgwick@utexas.edu

Abstract

Here, a reaction-based indicator displacement hydrogel assay (RIA) was developed for the detection of hydrogen peroxide (H₂O₂) via the oxidative release of the optical reporter Alizarin Red S (ARS). In the presence of H₂O₂, the RIA system displayed potent biofilm inhibition for Methicillin-resistant Staphylococcus aureus (MRSA), as shown through an in vitro assay quantifying antimicrobial efficacy. This work demonstrated the potential of H₂O₂-responsive hydrogels containing a covalently bound diol-based drug for controlled drug release.

This article is part of the themed collection: The Mechanics of Supramolecular Chemistry

Chemical Communications

Reaction-based indicator displacement assay (RIA) for the development of a triggered release system capable of biofilm inhibition†

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Reaction-based indicator displacement assay (RIA) for the development of a triggered release system capable of biofilm inhibition

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