Issue 17, 2019

A novel “AIE + ESIPT” near-infrared nanoprobe for the imaging of γ-glutamyl transpeptidase in living cells and the application in precision medicine

Abstract

γ-Glutamyl transpeptidase (GGT) has been reported as a biomarker of hepatocellular carcinoma (HCC), and its imaging is of great benefit for early detection in precise medicine as well as intraoperative navigation. Herein, we have designed and synthesized a novel near-infrared fluorescent probe coupled aggregation-induced emission (AIE) and excited-state intramolecular proton transfer (ESIPT) effect for the detection of GGT. Thanks to conjugated glutamate acid, this probe could be dispersed in aqueous solution and showed barely any fluorescence emission. Through a GGT-mediated enzymatic reaction, the aggregation state of the probe in aqueous solution was changed and an intramolecular hydrogen bond was formed, resulting in an enhanced fluorescence emission. An excellent linear relationship was observed and the concentration of GGT measured was in the range of 10–90 U L−1 with a limit of detection calculated at 2.9 U L−1. Its feasibility has been confirmed by detecting GGT in HepG2 cells with high specificity and long-term sustainability, satisfying clinical need. Moreover, this nanoprobe showed great potential for precise medicine guided surgery by realizing fluorescence imaging in human liver tumour tissue and distinguishing it from normal tissue. Thus, we supposed that our AIE coupled ESIPT fluorescent nanoprobe has great potential in the early detection of HCC, the selective fluorescence imaging of GGT positive cells during surgery and application in precision medicine.

Graphical abstract: A novel “AIE + ESIPT” near-infrared nanoprobe for the imaging of γ-glutamyl transpeptidase in living cells and the application in precision medicine

Supplementary files

Article information

Article type
Paper
Submitted
29 avr. 2019
Accepted
19 juin 2019
First published
03 juil. 2019

Analyst, 2019,144, 5136-5142

A novel “AIE + ESIPT” near-infrared nanoprobe for the imaging of γ-glutamyl transpeptidase in living cells and the application in precision medicine

Y. Liu, B. Feng, X. Cao, G. Tang, H. Liu, F. Chen, M. Liu, Q. Chen, K. Yuan, Y. Gu, X. Feng and W. Zeng, Analyst, 2019, 144, 5136 DOI: 10.1039/C9AN00773C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements