Issue 88, 2017

Recognition-driven chemical labeling of endogenous proteins in multi-molecular crowding in live cells

Abstract

Endogenous protein labeling is one of the most invaluable methods for studying the bona fide functions of proteins in live cells. However, multi-molecular crowding conditions, such as those that occur in live cells, hamper the highly selective chemical labeling of a protein of interest (POI). We herein describe how the efficient coupling of molecular recognition with a chemical reaction is crucial for selective protein labeling. Recognition-driven protein labeling is carried out by a synthetic labeling reagent containing a protein (recognition) ligand, a reporter tag, and a reactive moiety. The molecular recognition of a POI can be used to greatly enhance the reaction kinetics and protein selectivity, even under live cell conditions. In this review, we also briefly discuss how such selective chemical labeling of an endogenous protein can have a variety of applications at the interface of chemistry and biology.

Graphical abstract: Recognition-driven chemical labeling of endogenous proteins in multi-molecular crowding in live cells

Article information

Article type
Feature Article
Submitted
13 sept. 2017
Accepted
05 oct. 2017
First published
13 oct. 2017

Chem. Commun., 2017,53, 11972-11983

Recognition-driven chemical labeling of endogenous proteins in multi-molecular crowding in live cells

K. Amaike, T. Tamura and I. Hamachi, Chem. Commun., 2017, 53, 11972 DOI: 10.1039/C7CC07177A

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