Issue 3, 2015
From the journal:

Chemical Communications

A thermostable transketolase evolved for aliphatic aldehyde acceptors†

Dong Yi,a   Thangavelu Saravanan,a   Titu Devamani,a   Franck Charmantray,b   Laurence Hecquetb  and  Wolf-Dieter Fessner*a  

* Corresponding authors

a Institut für Organische Chemie und Biochemie, Technische Universität Darmstadt, Alarich-Weiss-Str. 4, 64287 Darmstadt, Germany
E-mail: fessner@tu-darmstadt.de
Fax: +49 6151 166636
Tel: +49 6151 166666

b Clermont Université, Université Blaise Pascal, Institut de Chimie de Clermont-Ferrand, CNRS UMR 6296, ICCF, BP10448, 63177 Aubière, France

Abstract

Directed evolution of the thermostable transketolase from Geobacillus stearothermophilus based on a pH-based colorimetric screening of smart libraries yielded several mutants with up to 16-fold higher activity for aliphatic aldehydes and high enantioselectivity (>95% ee) in the asymmetric carboligation step.

Graphical abstract: A thermostable transketolase evolved for aliphatic aldehyde acceptors

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Article information

DOI
https://doi.org/10.1039/C4CC08436E
Article type
Communication
Submitted
25 oct. 2014
Accepted
18 nov. 2014
First published
18 nov. 2014
This article is Open Access
Creative Commons BY license

Chem. Commun., 2015,51, 480-483

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A thermostable transketolase evolved for aliphatic aldehyde acceptors

D. Yi, T. Saravanan, T. Devamani, F. Charmantray, L. Hecquet and W. Fessner, Chem. Commun., 2015, 51, 480 DOI: 10.1039/C4CC08436E

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