Luminescence detection of peptide:N-glycanase activity using engineered split inteins

Tsuyoshi Takahashi; Tatsuya Uchibayashi; Nozomi Ishii; Ichiro Matsuo; Yukiko Yoshida; Tadashi Suzuki

doi:10.1039/D2CC04865E

You do not have JavaScript enabled. Please enable JavaScript to access the full features of the site or access our non-JavaScript page.

Luminescence detection of peptide:N-glycanase activity using engineered split inteins†

Tsuyoshi Takahashi,

*^a Tatsuya Uchibayashi,^a Nozomi Ishii,^a Ichiro Matsuo,

^a Yukiko Yoshida

^b and Tadashi Suzuki^c

Author affiliations

* Corresponding authors

^a Graduate School of Science and Technology, Gunma University, 1-5-1 Tenjin-cho, Kiryu, Gunma 376-8515, Japan
E-mail: ttakahas@gunma-u.ac.jp

^b Ubiquitin Project, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo 156-8506, Japan

^c Glycometabolic Biochemistry Laboratory, RIKEN Cluster for Pioneering Research, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan

Abstract

A split intein-based method has been developed to detect peptide:N-glycanase (PNGase) activity in live cells. PNGase cleaves the linkage between N,N′-diacetylchitobiose and the Asn side-chain of N-intein peptides and the products react rapidly with C-intein by protein trans-splicing to generate an active luciferase.

This article is part of the themed collection: Celebrating 10 years of ChemComm Emerging Investigators

Download options Please wait...

Supplementary files

Supplementary information PDF (1978K)

Article information

DOI: https://doi.org/10.1039/D2CC04865E
Article type: Communication
Submitted: 02 sept. 2022
Accepted: 07 nov. 2022
First published: 08 nov. 2022

Download Citation

Chem. Commun., 2022,58, 13282-13285

Permissions

Request permissions

Luminescence detection of peptide:N-glycanase activity using engineered split inteins

T. Takahashi, T. Uchibayashi, N. Ishii, I. Matsuo, Y. Yoshida and T. Suzuki, Chem. Commun., 2022, 58, 13282 DOI: 10.1039/D2CC04865E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Social activity

Fetching data from CrossRef.
This may take some time to load.

Chemical Communications

Luminescence detection of peptide:N-glycanase activity using engineered split inteins†

Abstract

Supplementary files

Article information

Download Citation

Permissions

Luminescence detection of peptide:N-glycanase activity using engineered split inteins

Social activity

Search articles by author

Spotlight

Advertisements