Issue 28, 2021

Development of manganese dioxide-based nanoprobes for fluorescence detection and imaging of glutathione

Abstract

Tripeptide glutathione (GSH) is an abundant and ubiquitous metabolite in living organisms and plays critical roles in various cellular processes. In this work, we report the development of a new nanoprobe (MnO2–PEI–FITC) for GSH detection and imaging by exploiting the response mechanism of specific GSH-triggered reduction of manganese dioxide (MnO2) nanosheets. The MnO2–PEI–FITC nanoprobe was developed by coating negatively charged MnO2 nanosheets with a positively charged polyethylenimine (PEI) polymer, followed by coupling with fluorescein isothiocyanate (FITC) through a thiourea linkage. The MnO2–PEI–FITC nanoprobe showed weak fluorescence due to the quenching of FITC emission absorption by MnO2, while the emission of FITC at 518 nm was observed in the presence of GSH. The MnO2–PEI–FITC nanoprobe is featured with a rapid response to GSH (<12 min), high sensitivity (detection limit, 164 nM) and selectivity. The application of this nanoprobe for GSH imaging in fresh yeast cells and onion inner-layer epidermal tissues was then successfully demonstrated. This work thus provides a new nanoprobe for GSH detection and imaging in biological samples.

Graphical abstract: Development of manganese dioxide-based nanoprobes for fluorescence detection and imaging of glutathione

Supplementary files

Article information

Article type
Paper
Submitted
15 avr. 2021
Accepted
08 juin 2021
First published
08 juin 2021

New J. Chem., 2021,45, 12377-12383

Development of manganese dioxide-based nanoprobes for fluorescence detection and imaging of glutathione

A. Qaitoon, J. Yong, Z. Zhang, J. Liu, Z. P. Xu and R. Zhang, New J. Chem., 2021, 45, 12377 DOI: 10.1039/D1NJ01843D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements