Issue 46, 2020

Förster resonance energy transfer (FRET) and applications thereof

Abstract

FRET is a nonradiative process of energy transfer that is based on the dipole–dipole interactions between molecules that are fluorescent. Transfer of energy takes place rapidly from a donor molecule to an acceptor molecule in juxtaposition such as 0 to 10 nm without photonic radiation. FRET has occupied a center stage in biotechnology and biological studies. It is used to gain information on conformation changes in single molecules. The pharmaceutical industry has also developed large fluorescence detection systems with very small sample sizes, at the level of single molecules, using fluorescence correlation spectroscopy.

Graphical abstract: Förster resonance energy transfer (FRET) and applications thereof

Associated articles

Article information

Article type
Critical Review
Submitted
20 oct. 2020
Accepted
29 oct. 2020
First published
19 nov. 2020

Anal. Methods, 2020,12, 5532-5550

Förster resonance energy transfer (FRET) and applications thereof

A. Kaur, P. Kaur and S. Ahuja, Anal. Methods, 2020, 12, 5532 DOI: 10.1039/D0AY01961E

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