Issue 10, 2019

Droplet-based single cell RNAseq tools: a practical guide

Abstract

Droplet based scRNA-seq systems such as Drop-seq, inDrop and Chromium 10X have been the catalyst for the wide adoption of high-throughput scRNA-seq technologies in the research laboratory. In order to understand the capabilities of these systems to deeply interrogate biology; here we provide a practical guide through all the steps involved in a typical scRNA-seq experiment. Through comparing and contrasting these three main droplet based systems (and their derivatives), we provide an overview of all critical considerations in obtaining high quality and biologically relevant data. We also discuss the limitations of these systems and how they fit into the emerging field of Genomic Cytometry.

Graphical abstract: Droplet-based single cell RNAseq tools: a practical guide

Supplementary files

Article information

Article type
Perspective
Submitted
11 nov. 2018
Accepted
04 avr. 2019
First published
08 avr. 2019

Lab Chip, 2019,19, 1706-1727

Droplet-based single cell RNAseq tools: a practical guide

R. Salomon, D. Kaczorowski, F. Valdes-Mora, R. E. Nordon, A. Neild, N. Farbehi, N. Bartonicek and D. Gallego-Ortega, Lab Chip, 2019, 19, 1706 DOI: 10.1039/C8LC01239C

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