A fluorescent peptidyl substrate for visualizing peptidyl-prolyl cis/trans isomerase activity in live cells

Quan Jiang; Xiao-Rong Li; Cheng-Kun Wang; Juan Cheng; Chao Tan; Tian-Tian Cui; Nan-Nan Lu; Tony D. James; Feng Han; Xin Li

doi:10.1039/C7CC09135D

A fluorescent peptidyl substrate for visualizing peptidyl-prolyl cis/trans isomerase activity in live cells†

Quan Jiang,‡^a Xiao-Rong Li,‡^a Cheng-Kun Wang,^a Juan Cheng,^a Chao Tan,^a Tian-Tian Cui,^a Nan-Nan Lu,^a Tony D. James,

^b Feng Han*^a and Xin Li

*^a

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* Corresponding authors

^a College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China
E-mail: changhuahan@zju.edu.cn, lixin81@zju.edu.cn

^b Department of Chemistry, University of Bath, Bath, UK

Abstract

This communication reports on a fluorescent probe (PPI-P) for imaging active peptidyl-prolyl cis/trans isomerases in live cells. PPI-P is capable of responding to both recombinant and cellular PPIases fluorogenically, and has been shown to specifically image active PPIases in live cells.

This article is part of the themed collection: The Mechanics of Supramolecular Chemistry

Chemical Communications

A fluorescent peptidyl substrate for visualizing peptidyl-prolyl cis/trans isomerase activity in live cells†

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A fluorescent peptidyl substrate for visualizing peptidyl-prolyl cis/trans isomerase activity in live cells

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