Issue 38, 2018

Methods for the detection of reactive oxygen species

Abstract

There are many pieces of evidence suggesting the mediating role of reactive oxygen species (ROS) in cell life, stress and death, and it is known that ROS at different concentrations would play distinctly different roles therein, thus eventually leading to the different fates of cells. Therefore it is highly necessary to carry out a reliable measurement of the concentration or relative level of ROS. Many means are available for ROS detection, such as the classical and commonly used spectrophotometry methods, use of fluorescence and chemiluminescence probes, and electron spin resonance (ESR/EPR), which provide direct identification of different types of oxygen radicals. In addition, fluorescent protein-based redox indicators designed for monitoring redox status changes would also be introduced in the text. Considering the fact that each type of method mentioned above has drawbacks during application, the present review tries to make a comparison and summary of their advantages and disadvantages systemically, and as a conclusion, the rational use of more than one method is recommended for better testing of the free radicals in cells.

Graphical abstract: Methods for the detection of reactive oxygen species

Article information

Article type
Critical Review
Submitted
14 juin 2018
Accepted
03 sept. 2018
First published
04 sept. 2018

Anal. Methods, 2018,10, 4625-4638

Methods for the detection of reactive oxygen species

Y. Zhang, M. Dai and Z. Yuan, Anal. Methods, 2018, 10, 4625 DOI: 10.1039/C8AY01339J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements