Issue 26, 2022

Cadherin-dependent adhesion modulated 3D cell-assembly

Abstract

The emergence of synthetic biology has opened new avenues in constructing cell-assembly biosystems with specific gene expression and function. The phenomena of cell spreading and detachment during tissue development and cancer metastasis are caused by surface tension, which in turn results from differences in cell–cell adhesion mediated by the dimerization of cadherin expressed on the cell surface. In this study, E- and P-cadherin plasmids were first constructed based on the differential adhesion hypothesis, then they were electroporated into K562 cells and HEK293T cells, respectively, to explore the process of cell migration and assembly regulated by cadherins. Using this approach, some special 3D cell functional components with a phase separation structure were fabricated successfully. Our work will be of potential application in the construction of self-assembling synthetic tissues and organoids.

Graphical abstract: Cadherin-dependent adhesion modulated 3D cell-assembly

Supplementary files

Article information

Article type
Paper
Submitted
09 may. 2022
Accepted
08 jun. 2022
First published
08 jun. 2022

J. Mater. Chem. B, 2022,10, 4959-4966

Cadherin-dependent adhesion modulated 3D cell-assembly

W. Han, M. He, Y. Zhang, J. Zhou, Z. Li, X. Liu, X. Sun, X. Yin, D. Yao and H. Liang, J. Mater. Chem. B, 2022, 10, 4959 DOI: 10.1039/D2TB01006B

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