Incorporation of selenium into selenoprotein P and extracellular glutathione peroxidase: HPLC-ICPMS data with enriched selenite

Abstract

The metabolic turnover of selenoprotein P (Sel P) and extracellular glutathione peroxidase (eGPx) in plasma was examined by HPLC-ICPMS, with the use of enriched Se, [⁸²Se]selenite. [⁸²Se]selenite was injected intravenously into rats at a dose of 25 µg ⁸²Se kg^–1 body weight, and the concentrations of labeled ⁸²Se and naturally occurring ⁷⁷Se in the serum, liver and kidneys were determined in samples obtained 1, 3, 6, 9, 12, 18 and 24 h after the injection. The distributions of both exogenous (labeled) ⁸²Se and endogenous (naturally occurring) ⁷⁷Se in serum, and supernatant fractions of the liver and kidneys were determined on a gel filtration column by HPLC-ICPMS. This dose was shown not to affect the constitutive levels of cellular GPx (cGPx), eGPx and Sel P. The labeled Se in Sel P increased soon after the injection, peaked at 6–9 h and then decreased, whereas that in eGPx continued increasing after 6 h post-injection and then throughout the remaining observation period in the bloodstream. These observations demonstrated the rapid and efficient incorporation of Se into Sel P in the liver and excretion into the plasma followed by the slow and steady incorporation of Se into eGPx in the kidneys and excretion into the plasma, with a minimal response of cGPx to selenite injection.